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1998 Fiscal Year Final Research Report Summary

Functional analysis of ionic channel and transporter of the cochlear marginal cell using cellular physiology and molecular biology

Research Project

Project/Area Number 09470367
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

IKEDA Katsuhisa  Medical School, Tohoku University Assistant Professor, 医学部, 講師 (70159614)

Co-Investigator(Kenkyū-buntansha) OSHIMA Takeshi  Medical School, Tohoku University Lecturer, 医学部, 助手 (40241608)
Project Period (FY) 1997 – 1998
KeywordsChannel / Transporter / Cochlea / Hearing / In-situ hybridization / RT-PCR / mRNA / Strial Vascularis
Research Abstract

A variety of ionic channels and transportes are known to be related to inner ear function. We have so far found Cl voltage-dependent chloride channels (ClC), Na-K-2C1 cotransporter (CCC), and Na-Ca exchanger (NCX), and Na-H exchange (NHE) in the cochlea by physiological methods. Furthermore, recent advances in molecular biology enabled us to prove the definite evidence of molecular characterization of these ion chanells and transportes. We used the reverse transcription-polymerase chain reaction (RT-PCR) to assess the expression of various isoforms and the non-radioactive in situ hybridization (ISH) to examine their localization. Among CIC family, ClC-2 and -3 were significantly present in the inner ear tissue. BSC2 transcript alone was detected in the cochlea and intensively located in the strial marginal cells and spiral ligament fibrocytes, indicating a good explanation of loop-diuretics cochleotoxicity. All of three isoforms of NCX (1-3) were detected in the inner ear. Unique spicing varients of NCX-1 were found in the cochlear modiolus. Although NHE-2, -3, and -4 isoform mRNAs could be detected in the cochlear tissue, NHE-1 message was clearly dominant. NHE-1 isoform expression was distributed in the hair cells, marginal cells, and spiral ligament fibrocytes, and ganglion cells.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ara HA et al: "Acute effects of combined administration of Kanaryuin" Tohoku J Exp Med. 186. 79-86 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oshima T et al: "Hearing loss with a mitochendnial gene" Laryngoscope. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goto S et al: "Expression and lscalization of Na-H exchange" Hear Res. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateda M et al: "pH regulation of the globular substance" Hear Res. 124. 91-98 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng et al: "Apoptotio changes in the aged cochleg" Hear Res. 126. 11-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda N et al: "Mitochmdrial DNA delation" Laryngoscope. 108. 580-584 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki H,Ikeda K,Takasaka T.: "Age-related changes of the globular substance in the otoconial membrane of mice." Laryngoscope. 107. 378-381 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oshima T,Ikeda K,Takasaka T.: "Expression of voltage-dependent chloride channels in the rat cochlea" Hear Res.103. 63-68 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda K,Oshima T,Hidaka H,Takasaka T.: "Molecular and clinical implications of loop diuretic ototoxicity." Hear Res.107. 1-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oshima T,Ikeda K,Furukawa M,Takasaka T.: "Alternatively spliced isoforms of the Na/Ca exchanger in the guinea pig cochlea." Biochem Biophys Res.Commun. 233. 737-741 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto S,Oshima T,Ikeda K,Ueda N,Takasaka T.: "Expression and localization of the Na-K-2Cl cotransporter in the mammalian cochlea." Brain Res.765. 324-326 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki H,Ikeda K,Furukawa M,Takasaka T.: "P_<2y> purinergic receptor of the globular substance in the otoconial membrane of the guinea pig inner ear." Am J Physiol.273. C1533-C1540 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda N,Oshima T,Ikeda K,Abe K,Aoki M,Takasaka T.: "Mitochondrial DNA deletion is a predisposing cause for sensorineural hearing loss." Laryngoscope. 108. 580-584 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawase T,Hidaka H,Ikeda K,Hashimoto S,Takasaka T.: "Acoustic reflex threshold and loudness in patients with unilateral hearing losses." Eur Arch Otorhinolaryngol. 255. 7-11 (1998)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Zheng Y,Ikeda K,Nakamura M,Takasaka T: "Apoptotic changes in the aged cochlea of Mongolian gerbil." Hear Res.126. 11-18 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateda M,Suzuki H,Ikeda K,Takasaka T: "pH regulation of the globular substance in the otoconial membrane of the guinea-pig inner ear." Hear Res.124. 91-98 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto S,Oshima T,Ikeda K,Takasaka T: "Expression and localizaton of teh Na-H^+ exchanger in the guinea pig cochlea." Hear Res.128. 89-96 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirano, K,Ikeda K,Kawase T,Oshima T,Kakehata S,Takahashi S,Sato T,Kobayashi T,Takasaka T: "Prognosis of sudden deafness with special reference to risk factors of microvascular pathology." ANL. (in perss.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oshima T,Ueda N,Ikeda K,Abe K,Takasaka T.: "Hearing loss with a mitochondrial gene mutation is highly prevalent in Japan." Laryngoscope. 109. 334-338 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ara HA,Ikeda K,Kawase K,Kukuchi T,Katori Y,Watanabe K,Takasaka T.: "Acute effects of combined administration of kanamycin and furosemide on the stria vascularis studied by distortion product otoacoustic emission and transmission electron microscopy." Tohoku J Exp Med. 186. 79-86 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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