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2000 Fiscal Year Final Research Report Summary

Immuno-histochemical and microbiological study on the relationships between microcirculatory system and immuno-defence system in the dental pulp.

Research Project

Project/Area Number 09470389
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YOSHIDA Shigemitsu  Hokkaido Univ.Grad.Sch.Dent.Med.Prof., 大学院・歯学研究科, 教授 (80174928)

Co-Investigator(Kenkyū-buntansha) SAWA Yoshihiko  Hokkaido Univ.Grad.Sch.Dent.Med.Asso.Prof., 大学院・歯学研究科, 助教授 (70271666)
SHIBATA Kenichiro  Hokkaido Univ.Grad.Sch.Dent.Med.Asso.Prof., 大学院・歯学研究科, 助教授 (50145265)
WATANABE Tsuguo  Hokkaido Univ.Grad.Sch.Dent.Med.Prof., 大学院・歯学研究科, 教授 (10064362)
YAMAOKA Yuji  Hokkaido Univ.Grad.Sch.Dent.Med.Inst., 大学院・歯学研究科, 助手 (50322821)
MAKINO Shujiroh  Hokkaido Univ.Dent.Hospital Inst., 歯学部・附属病院, 助手 (30280853)
Project Period (FY) 1997 – 2000
KeywordsDental pulp / Immuno-defence system / Leukocyte adhesion molecule / Lymphatic vessel / Immuno-histochemistry / PECAM-1 / ICAM-1 / ELAM-1
Research Abstract

To clarify the relationships between microcirculatory system and immuno-defence system in the dental pulp, we examined human blood and lymphatic vessels immuno-histochemically and microbiologically. The results are summarized as follows :
1)In general, lymphatic vessels in healthy tissue express PECAM-1 only, but in inflamed tissue, they express PECAM-1, ICAM-1, ICAM-3, and VCAM-1.
2) In the submandibular lymph node, lymphatic vessels express PECAM-1 and ICAM-1.
3) In the tongue, lymphatic capillaries close to oral epithelitum express PECAM-1, ICAM-1, and ELAM-1, or PECAM-1 and ICAM-1. However, collecting lymphatic vessels express PECAM-1 only.
4) Mediation of inflammatory cytokines induce strong expression of PECAM-1, and expression of ICAM-1 on the lymphatic vessels.
5) Some oral microbial associates induce expression of leukocyte adhesion molecules and production of cytokines in the vascular endothelial cells.
6) In blood vessels of healthy dental pulp, there are strong expression of PECAM-1 and weak expression of MHC class II, ELAM-1, and ICAM-1.
7) In blood vessels of inflamed dental pulp, there are strong expression of PFCAM-1, MHC class II, ELAM-1, ICAM-1, ICAM-3, and VCAM-1.
8) In lymphatic vessels of healthy and inflamed dental pulp, there are strong expression of PECAM-1 and weak expression of ICAM-1.
9) PECAM-1 expression of the blood vessels was observed only on the luminal surface of the endothelium, but in the lymphatic vessels, it was found both on the luminal and abluminal surfaces of the endothelium.
10) Expression of desmoplakin is observed only in the lymphatic vessels, but not in the blood vessels, indicating that anti-desmoplakin antibody is useful to discriminate lymphatic vessels from blood vessels.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Sawa,Y.,Yoshida,S.,Shibata,K.,Suzuki,M.and Mukaida,A.: "Vascular endothelium of human dental pulp expresses diverse adhesion molecules for leukocyte emigration."Tissue and Cell. 30(2). 281-291 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa,Y.,Yoshida,S.,Ashikaga,Y.,Kim,T.,Yamaoka,Y.and Shiroto,H.: "Lymphatic endothelium expresses PECAM-1."Tissue and Cell. 30(3). 377-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa,Y.,Yoshida,S.,Ashikaga,Y.,Kim,T.,Yamaoka,Y.and Suzuki,M.: "Immuno-histochemical demonstration of lymphatic vessels in human dental pulp."Tissue and Cell. 30(5). 510-517 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa,Y.,Shibata,K.,Braithwaite,M.W.,Suzuki,M.and Yoshida,S.: "Expression of immunoglobulin superfamily members on the lymphatic endothelium of inflamed human small intestine."Microvascul.Res.. 57(2). 100-106 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ebata,N.,Sawa,Y.,Ashikaga,Y.,Yamaoka,Y.,Suzuki,M.,Totsuka,Y.and Yoshida,S.: "Lymphatic endothelium of the human tongue expresses multiple leukocyte adhesion molecules."Tissue and Cell. 31(1). 34-38 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa,Y.,Yamaoka,Y.,Ebata,N.,Ashikaga,Y,Kim,T.,Suzuki,M.and Yoshida,S.: "Immunohistochemical study on leukocyte adhesion molecules expressed on lymphatic endothelium."Microvascul.Res.. 57(3). 292-297 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dong,L.,Shibata,K.,Sawa,Y.,Hasebe,A.,Yamaoka,Y.,Yoshida,S.and Watanabe,T.: "Transcriptional activation of mRNA of intercellular adhesion molecule 1 and induction of its surface expression in normal human gingival fibroblasts by Mycoplasma salivalium and M.fermentans."Infect.Immun.. 67(6). 3061-3065 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ebata,N.,Nodasaka,Y.,Sawa,Y.,Yamaoka,Y.,Makino,S.,Totsuka,Y.,Yoshida,S.: "Desmoplakin as a specific marker of lymphatic vessels."Microvascul.Res.. 61(1),. 40-48 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ebata,N.,Sawa,Y.,Nodasaka,Y.,Yamaoka,Y.,Yoshida,S.,Totsuka,Y.: "Immuno-electron microscope study of PECAM-1 expression on the lymphatic endothelium of human tongue."Tissue and Cell. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa, Y., Yoshida, S., Shibata, K., Suzuki, M.and Mukaida, A.: "Vascular endothelium of human dental pulp expresses diverse adhesion molecules for leukocyte emigration."Tissue and Cell. 30(2). 281-291 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawa, Y., Yoshida, S., Ashikaga, Y., Kim, T., Yamaoka, Y.and Shiroto, H.: "Lymphatic endothelium expresses PECAM-1."Tissue and Cell. 30(3). 377-382 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawa, Y., Yoshida, S., Ashikaga, Y., Kim, T., Yamaoka, Y.and Suzuki, M.: "Immuno-histochemical demonstration of lymphatic vessels in human dental pulp."Tissue and Cell. 30(5). 510-517 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawa, Y., Shibata, K., Braithwaite, M.W., Suzuki, M.and Yoshida, S.: "Expression of immunoglobulin superfamily members on the lymphatic endothelium of inflamed human small intestine."Microvascul.Res.. 57(2). 100-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ebata, N., Sawa, Y., Ashikaga, Y., Yamaoka, Y., Suzuki, M., Totsuka, Y.and Yoshida, S.: "Lymphatic endothelium of the human tongue expresses multiple leukocyte adhesion molecules."Tissue and Cell. 31(1). 34-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawa, Y., Yamaoka, Y., Ebata, N., Ashikaga, Y., Kim, T., Suzuki, M.and Yoshida, S.: "Immunohistochemical study on leukocyte adhesion molecules expressed on lymphatic endothelium."Microvascul.Res.. 57(3). 292-297 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dong, L., Shibata, K., Sawa, Y., Hasebe, A., Yamaoka, Y., Yoshida, S.and Watanabe, T.: "Transcriptional activation of mRNA of intercellular adhesion molecule 1 and induction of its surface expression in normal human gingival fibroblasts by Mycoplasma salivalium and M.fermentans."Infect.Immun.. 67(6). 3061-3065 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ebata, N., Nodasaka, Y., Sawa, Y., Yamaoka, Y., Makino, S., Totsuka, Y., Yoshida, S.: "Desmoplakin as a specific marker of lymphatic vessels."Microvascul.Res.. 61(1). 40-48 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ebata, N., Sawa, Y.ÅC Nodasaka, Y., Yamaoka, Y., Yoshida, S., Totsuka, Y.: "Immuno-electron microscope study of PECAM-1 expression on the lymphatic endothelium of human tongue."Tissue & Cell. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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