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1999 Fiscal Year Final Research Report Summary

Predominant culture-difficult bacteria in periodontal pockets and their pathogenicity.

Research Project

Project/Area Number 09470390
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

HOSHINO Etsuro  Niigata University School of Dentistry Professor, 歯学部, 教授 (90124619)

Co-Investigator(Kenkyū-buntansha) SATO Naomi  School of Dentistry, Niigata University Assistant, 歯学部, 助手 (20018427)
NAKAZAWA Futoshi  School of Dentistry, Niigata University Assistant Professor, 歯学部, 助教授 (60115053)
Project Period (FY) 1997 – 1999
Keywordsculture-difficult bacteria / periodonttitis / asaccharolytic bacteria / oral anaerobes / γδ T cell / IL-4 / IFNィイD2γィエD2
Research Abstract

The present project aimed to clarify the pathogenic factors of asaccharolytic anaerobic Gram-positive rods (AAGPR). AAGPR are so culture-difficult that they had been ignored until we reported they were predominant in various oral florae when excellent strict anaerobic procedures were adapted. Most of them are not classified to any established bacterial genera and species, thus, another aim is to clarified their phylogenetic positions.
1. Groups of clinical isolates were assigned to 2 new bacterial genera, Cryptobacterium and Mogibacterium, and 4 new species according to the 16SrDNA sequence analysis results.
2. PCR method may be useful for the detection of culture-difficult bacteria, so that species-specific primers of AAGPR were designated, and in fact, they were detected from various oral sites.
3. When AAGPR were injected into mouse peritoneal cavities, γδ_ cell responded primary to produce IL-4 and IFNィイD2γィエD2, indicating they induced humoral and cellular immunities. The immune reactions were as strong as those induced by Porphyromonas gingivalis injected intraperioneally as a control bacteria, suggesting AAGPR possess high immunological potency and play an important role in periodontitis as predominant bacteria in periodontal pockets.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Nakazawa F: "Description of Mogibacterium pumilum gen. nov., sp. nov."International Journal of Systematic and Evolutionary Microbiology. 50(2). 679-688 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hori R: "Tongue microflora in edentulous geriatric denture-wearers"Microbial Ecology in Health and Diseases. 11. 89-95 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa F: "Crytobacterium curtum gen. nov., sp. nov., a new genus of Gram-positive anaerobic rod isolated from human oral cavities"International Journal of Systematic Bacteriology. 49(3). 1193-1200 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MD. ali Asgor Moral: "Experimantal chronic infection induced in mice by Actinomyces israelii entrapped by alginate gel."Archives of oral biology. 43. 485-496 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumita M: "Experimental actinomycosis in mice using alginate gel particles containing Actinomyces israelli"Endodontics and Dental Traumatology. 14. 137-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato T: "Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism"Archives of oral Biology. 43. 247-252 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato T: "Restricted fragment-length polymorphism analysis of 16SrDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction"Oral Microbiology and Immunology. 13. 23-29 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa F: "Genetic characterization of an oral treponeme isolated from human subgingival plaque"Oral Microbiology and Immunology. 12. 189-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umemoto T: "Treponema medium sp. nov., isolated from human subgingival dental plaque"International Journal of Systematic Bacteriology. 47. 67-72

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa F: "Description of Mogibacterium pumilum gen. nov., sp. nov. and Mogibacterium vescum gen. nov., sp. nov., and reclassification of Eubacterium timidum (Holdeman et al. 1980) as Mogibacterium timidum gen. nov., comb. nov."International Journal of Systematic and Evolutionary Microbiology. 50. 679-688 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hori R: "Tongue microflora in edentulous geriatric denture-wearers"Microbial Ecology in Health and Disease. 11. 89-95 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazawa F: "Cryptobacterium curtum gen. nov., sp. nov., a new of Gram-positive anaerobic rod isolated from human oral cavities."International Journal of Systematic genus Bacteriology. 49(3). 1193-1200 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MD. Ali Asgor Moral: "Experimantal chronic infection induced in mice. by Actinomyces israelii entrapped by alginate gel."Archives of oral Biology. 43. 485-496 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sumita M: "Experimental actinomycosis in mice using alginate gel particles containing Actinomyces israelii."Endodontics and Dental Traumatology. 14. 137-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato T: "Differentiation of oral Actinomyces species by 16S ribosomal. DNA polymerase chain reaction-restriction fragment length polymorphism"Archives of oral Biology. 43. 247-252 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato T: "Restricted fragment-length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction."Oral Microbiology and Immunology. 13. 23-29 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazawa F: "Genetic characterization of an oral treponeme isolated from human subgingival plaque."Oral Microbiology and Immunology. 12. 189-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umemoto T: "Treponema medium sp. nov., isolated from human subgingival dental plaque."International Journal of Systematic 【approximately equal】 Bacteriology. 47. 67-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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