• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

GST-p and p53 in 4NQO-induced tongue carcinomas of two strains of rats

Research Project

Project/Area Number 09470414
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionKagoshima University

Principal Investigator

KITANO Motoo  Kagoshima University Dental School, Professor, 歯学部, 教授 (10142118)

Co-Investigator(Kenkyū-buntansha) TAKASAKI Takashi  Kagoshima University Dental School, Research Associate, 歯学部, 助手 (60264438)
LI Tie-Jun  Kagoshima University Dental School, Research Associate, 歯学部, 助手 (70281227)
TANUMA Jun-ichi  Kagoshima University Dental School, Research Associate, 歯学部, 助手 (20305139)
SEMBA Ichiro  Kagoshima University Dental School, Assistant Professor, 歯学部, 講師 (60145505)
Project Period (FY) 1997 – 1998
Keywords4NQO / tongue carcinoma / DA rat / WF rat / Susceptible gene / Resistant gene / p53 / GST-p
Research Abstract

Immunohistochemical expression of p53 protein during rat lingual carcinogenesis was studied using two strains of rats [Dark-Agouti (DA) and Wistar/Furth (WF) rats] treated with 4-nitroquinoline 1-oxide (4NQO). Animal experiments were designed to look into differences in carcinogenesity between the two strains in two different fashions, i.e. 4NQO treatment being lasted until the moribund state or using a defined treatment schedule. Although the immunohistochemical results showed that the positive ratio of p53 protein in the established SCCs was similar between DA and WF rats, the clusters of p53 positive cells, usually situated in radom groups in the dysplastic lingual epithelium, were greater in numbers in DA rats than in WF.Reactivity of p53 in DA rats was detected earlier than that in WF, and marked increase of p53 positive clusters in DA rats was noted at the later stages of the experiment, whereas in WF rats the number of p53 positive clusters seemed to be unchanged. It was also demonstrated that DNA samples of the tongue tissues of 7 DA and 2 WF rats (13.4%) showed positive mutations in p53 gene. A very common gene mutation was observed in exon 5 (TGC _3 TAC transitions at codon 174) in all 9 rats. In summary, 64.3 % of the rats with immunohistochemically positive p53 protein in the tongue exhibited some p53 gene mutations. The remaining 35.7 % (5 DA rats) had p53 protein positive clusters in their lingual mucosae, however, they did not show any detectable p53 gene mutations. Thus, the present results may suggest p53 protein abnormalities play some important roles at the earlier stages of rat lingual carcinogenesis inspite of the absence of p53 gene mutations. These results also suggest that there may be strain differences in the p53 response to 4NQO between DA and WF rats, and these differences may contribute to the contrasting carcinogenesity.

  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] T-J Li,Y Hirayama,M Kitano: "Glutathione S-transferase π-class as a tumour marker in lingual preneoplastic and neoplastic lesions of rats and humans." Virchows Arch. 431. 37-43 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H Shisa,L Lu,H Katoh,A Kawarai,J Tanuma,Y Matsushima,H Hiai: "The LEXF: a new set of rat recombinant inbred strains between LE/Stm and F344." Mammalian Genome. 8. 324-327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano,H Mukai,S Yamashita: "Oral sebaceous carcinoma: report of a case." J Oral Maxillofac Surg. 55. 751-754 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano,A Yoshida,Y Iwashige,S Yamashita: "Myxoglobulosis in an extravasation mucocele of the lower lip." J Oral Pathol Med. 26. 342-344 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano,M Tsuneyoshi,S Sonoda,T Mimura: "Intra-articular fibroma of tendon sheath in the temporomandibular joint." Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 84. 407-410 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano: "Oriental Kimura's disease and its relation to angiolymphoid hyperplasia with eosinophilia(ALHE)." South Pacific Study. 17. 287-300 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano: "Epithelial cell proliferation in odontogenic keratocyst: a review." Ann Kagoshima University Dental School. 17. 27-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano: "Reviewing the unicystic ameloblastocma: a clinicopathologically distinct entity." Oral Med Pathol. 2. 61-68 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y Kuwashima,Y Kobayashi,A Kawarai,M Kurosumi,J Tanuma,K shiromizu,K Kishi: "Occurrence of apoptotic DNA fragmentation in quiescent and proliferating cells in human endometrial adenocarcinoma tissues and the influence of apoptosis-suppressing effects of bcl-2 products." Anticancer Res. 17. 3737-3742 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y Kuwashima,Y Kobayashi,M Kurosumi,J Tanuma,K Shiromizu,K Kishi: "Inverse correlation between bcl-2 expression and cell growth fraction in human endometrial adenocarcinoma tissue." Anticancer Res. 17. 3773-3776 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K Kitada,M Inoue,M Kitano: "Experimental endocarditis induction and platelet aggregation by Streptococcus anginosus, Streptococcus constellatsu and Streptococcus intermedius." FEMS Immunol Med Microbiol. 19. 25-32 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K Kubo,N Tsukasa,M Uehara,Y Izumi,M Orino,M Kitano,T Sueda: "Calcium and silicon from bioactive glass concerned with formation of nodules in periodontal-ligament fibroblasts in vitro." J Oral Rehabilit. 24. 70-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M Kitano,C E Lehner,Y Taya,H S Ozaki,M Takenaka,M Kawashima: "Histopathological study on dysbaric osteonecrosis(DON)in tibiae of sheep with a hyperbaric exposure." South Pacific Study. 18. 17-31 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M Kawashima,H Tamura,Y Noro,K Takao,K Yoshida,T Tsunosue,M Kitano,C E Lehner: "Diving profile and dysbaric osteonecrosis." High Pressure Biology and Medicine. 5. 185-194 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M Kitano,M Kawashima,Y Taya,C E Lehner: "Bone marrow changes in the tibiae of sheep with experimentally induced decompression sickness." High Pressure Biology and Medicine. 5. 195-205 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J Tanuma,H Shisa,H Hiai,S Higashi,Y Yamada,T Kamoto,Y Hirayama,H Matsuuchi,M Kitano: "Quantitative trait loci affecting 4NQO-induced tongue carcinogenesis in the rat." Cancer Res. 56. 1660-1664 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y Miura,H S Ozaki,T-J Li,M Umemura,M Kitano: "Experimental odontogenic cysts induced by in vitro 4-nitroquinoline 1-oxide (4NQO)treatment of F344 rat incisor tooth germs." J Oral Pathol Med. 27. 53-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano,X-M Chen,T Itoh,K Kawashima,K Sugihara,E Nozoe,T Mimura: "Orthokeratinized odontogenic cyst: a clinicopathological and immunocytochemical study of 15 cases." Histopathology. 32. 242-251 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li,M Kitano,K Arimura,K Sugihara: "Recurrence of unicystic ameloblastoma : a case report and review of the literature." Arch Pathol Lab Med. 122. 371-374 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y Kuwashima,M Kurosumi,Y Kobayashi,J Tanuma,K Suemasu,Y Higashi,T Kasamatsu,K Shiromizu,K Kishi: "Random nuclear p53 overexpression pattern in tamoxifen-mediated exndometrial carcinoma." Int J Gynecol Pathol. 17. 135-139 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K Takahashi,G H Nuckolls,O Tanaka,I Semba,I Takahashi,R Dashner,L Shum,H C Slavikin: "Adenovirus-mediated ectopic expression of Msx2 in even-numvered thombomeres induces apoptotic elimination of cranial neural crest cells in ovo." Development. 125. 1627-1635 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I Takahashi,G H Nuckolls,K Takahashi,O Tanaka,I Semba,R Dashner,L Shum,H C Slavkin: "Compressive force promotes Sox9,type II collagen and aggrecan and inhibits IL-1b expression resulting in chondrogenesis in mouse embryonic limb bud mesenchymal cells." J Cell Sci. 111. 2067-2076 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S Akifusa,M Ohguchi,T Koseki,K Nara,I Semba,K Yamato,N Okahashi,R Merino,B Nunez,N Hanada,T Takehara,T Nishihara: "Increase in Bcl-2 level promoted by CD40 ligation correlates with inhibition of B cell apoptosis induced by vacuolar type H+-ATPase inhibitor." Exper Cell Res. 238. 82-89 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y Kawabata,I Semba,Y Hirayama,T Koga,S Nagao,H Takada: "Wax D of Mycobacterium tuberculosis induced osteomyelitis accompanited with reactive bone formation in Buffalo rats." FEBS Immunol Med Microbiol. 22. 293-302 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H Kuniyoshi,M Kitano,Y Uchio T E Enosa,L F Aloaina,V F Asaua,S Terashi: "Seroepidemiological survey of anti-human T-cell leukemia virus-type I antibodies in Western Samoa." South Pacific Study. 18. 51-56 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T Takasaki,S Akiba,Y Sagara,H Yoshida: "Histological and biolobical characteristics of microinvasion in mammary carcinomas < 2cm in diameter." Pathol Intern. 48. 800-80 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T-J Li, Y Hirayama, M Kitano: "Glutathione S-transferase pi-class as a tumour marker in lingual preneoplastic and neoplastic lesions of rats and humans" Virchows Arch. 431. 37-43 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Shisa, L Lu, H Katoh, A Kawarai, J Tanuma, Y Matsushima, H Hiai: "The LEXF : a new set of rat recombinant inbred strains between LE/Stm and F344" Mammalian Genome. 8. 324-327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano, H Mukai, S Yamashita: "Oral sebaceous carcinoma : report of a case" J Oral Maxillofac Surg. 55. 751-754 (1977)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano, M Tsuneyoshi, S Sonoda, T Mimura: "Intra-articular fibroma of tendon sheath in the temporomandibular joint" Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 84. 407-410 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano: "Oriental Kimura's disease and its relation to angiolymphoid hyperplasia with eosinophilia (ALHE)" South Pacific Study. 17. 287-300 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano: "Epithelial cell proliferation in odontogenic keratocyst : a review" Ann Kagoshima University Dental School. 17. 27-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano: "Reviewing the unicystic ameloblastocma : a clinicopathologically distinct entity" Oral Med Pathol. 2. 61-68 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kuwashima, Y Kobayashi, A Kawarai, M Kurosumi, J Tanuma, K Shiromizu, K Kishi: "Occurrence of apoptotic DNA fragmentation in quiescent and proliferating cells in human endometrial adenocarcinoma tissues and the influence of apoptosis-suppressing effects of bcl-2 products" Anticancer Res. 17. 3737-3742 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kuwashima, Y Kobayashi, M Kurosumi, J Tanuma, K Shiromizu, K Kishi: "Inverse correlation between bcl-2 expression and cell growth fraction in human endometrial adenocarcinoma tissue" Anticancer Res. 17. 3773-3776 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Kitada, M Inoue, M Kitano: "Experimental endocarditis induction and platelet aggregation by Streptococcus anginosus, Streptococcus constellatus and Streptococcus intermedius" FEMS Immunol Med Microbiol. 19. 25-32 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Kubo, N Tsukasa, M Uehara, Y Izumi, M Orino, M Kitano, T Sueda: "Calcium and silicon from bioactive glass concerned with formation of nodules in periodontal-ligament fibroblasts in vitro" J Oral Rehabilit. 24. 70-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M Kitano, C E Lehner, Y Taya, H S Ozaki, M Takenaka, M Kawashima: "Histopathological study on dysbaric osteonecrosis (DON) in tibiae of sheep with a hyperbaric exposure" South Pacific Study. 18. 17-31 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M Kawashima, H Tamura, Y Noro, K Takao, K Yoshida, T Tsunosue, M Kitano: "C E Lehner.Diving profile and dysbaric osteonecrosis" High Pressure Biology and Medicine (Papers presented at the Vth International Meeting on High Pressure Biology). 185-194 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M Kitano, M Kawashima, Y Taya, C E Lehner: "Bone marrow changes in the tibiae of sheep with experimentally induced decompression sickness" High Pressure Biology and Medicine (Papers presented at the Vth International Meeting on High Pressure Biology). 195-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J Tanuma, H Shisa, H Hiai, S Higashi, Y Yamada, T Kamoto, Y Hirayama, H Matsuuchi, M Kitano: "Quantitative trait loci affecting 4NQO-induced tongue carcinogenesis in the rat" Cancer Res. 56. 1660-1664 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Miura, H S Ozaki, T-J Li, M Uemura, M Kitano: "Experimental odontogenic cysts induced by in vitro 4-nitroquinoline 1-oxide (4NQO) treatment of F344 rat incisor tooth germs" J Oral Pathol Med. 27. 53-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano X-M Chen, T Itoh, K Kawashima, K Sugihara, E Nozoe, T Mimura: "Orthokeratinized odontogenic cyst : a clinicopathological and immunocytochemical study of 15 cases" Histopathology. 32. 242-251 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T-J Li, M Kitano, K Arimura, K Sugihara: "Recurrence of unicystic ameloblastoma : a case report and review of the literature" Arch Pathol Lab Med. 122. 371-374 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kuwashima, M Kurosumi, Y Kobayashi, J Tanuma, K Suemasu, Y Higashi, T Kasamatsu, K Shiromizu, K Kishi: "Random nuclear p53 overexpression pattern in tamoxifen-mediated exndometrial carcinoma" Int J Gynecol Pathol. 17. 135-139 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Takahashi, G H Nuckolls, O Tanaka, I Semba, I Takahashi, R Dashner, L Shum, H C Slavikin: "Adenovirus-mediated ectopic expression of Msx2 in even-numbered thombomeres induces apoptotic elimination of cranial neural crest cells in ovo" Development. 125. 1627-1635 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I Takahashi, G H Nuckolls, K Takahashi, O Tanaka, I Semba, R Dashner, L Shum, H C Slavkin: "Compressive force promotes Sox9, type II collagen and aggrecan and inhibits IL-1b expression resulting in chondrogenesis in mouse embryonic limb bud mesenchymal cells" J Cell Sci. 111. 2067-2076 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Akifusa, M Ohguchi, T Koseki, K Nara, I Semba, K Yamato, N Okahashi, R Merino, B Nunez, N Hanada, T Takehara, T Nishihara: "Increase in Bcl-2 level promoted by CD40 ligation correlates with inhibition of B cell apoptosis induced by vacuolar type H^+-ATPase inhibitor" Exper Cell Res. 238. 82-89 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kawabata, I Semba, Y Hirayama, T Koga, S Nagao, H Takada: "Wax D of Mycobacterium tuberculosis induced osteomyelitis accompanited with reactive bone formation in Buffalo rats" FEBS Immunol Med Microbiol. 22. 293-302 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Kuniyoshi, M Kitano, Y Uchio, T E Enosa, L F Aloaina, V F Asaua, S Terashi: "Seroepidemiological survey of anti-human T-cell leukemia virus-type I antibodies in Western Samoa" South Pacific Study. 18. 51-56 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T Takasaki, S Akiba, Y Sagara, H Yoshida: "Histological and biolobical characteristics of microinvasion in mammary carcinomas < 2cm in diameter" Pathol Intern. 48. 800-805 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi