1998 Fiscal Year Final Research Report Summary
Corelation of IL-1B wnverting engyme with IL-1 production and Apoptosis in fibrobla
| Project/Area Number |
09470428
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | SHOWA UNIVERSITY |
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Principal Investigator |
KOBAYASHI Makato Showa University,School of Dentistry lecturer, 医学部, 講師 (80186767)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIHARA Tatsuji National Institute of Infections Diseases head, 室長 (80192251)
MIYAZAWA Yasushi Showa University,School of Dentistry lecturer, 医学部, 講師 (90219775)
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| Project Period (FY) |
1997 – 1998
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| Keywords | gingival fibroblast / IL-1 / ICE / apoptosis / Caspase |
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| Research Abstract |
In this study, we determined whether human recombinant interleukin1 (IL-1) stimulated human gingival fibroblast (HGF) to expressionIL-1s (IL-1α, IL-1β, IL-1 receptor, IL-1 receptor antagonist) and IL-1 converting enzyme (ICE). In addition, we have studied the secretion and processing of the two forms IL-1(α and β) from IL-1 induced HGF.
In agreement with previous results, we have found that no processed IL-1(α and β) is detected in culture supernatant. And we have used western blot analysis and ELISA system experiment to follow the processing of IL-1 in HGF. Most of the pro-IL-1 α and β remains in cytoplasm, some of the pro-IL-1 cleavage and transported mature form to the cell membrane. IL-1 (α or β) stimulated HGF IL-1s mRNA accumulation, did not ICE mRNA. And we have no detected caspase-1 activity in IL-1-induced HGF. These findings suggested that ICE mRNA accumulation was not associated with HGF production of mature IL-1β protein.
While we investigated meditative role of caspase in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans. We found that the activation of caspases, in particular, caspase-3, was involved in the apoptotic cell death of macrophages. We have demonstrated that the caspase-1 is transiently activated, whereas the caspase-3 is gradually activated during the cell infected with bacteria. They suggested caspase may an important role in survival of monocytes apoptotic regulation.
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