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1999 Fiscal Year Final Research Report Summary

A study on the role of nitric oxide in the response of periodontal tissue to mechanical stimuli

Research Project

Project/Area Number 09470465
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MITANI Hideo  School of Dentistry, Tohoku University, Professor, 歯学部, 教授 (50014220)

Co-Investigator(Kenkyū-buntansha) SAEKI Shunichi  Dental Hospital, Instructor, 歯学部・附属病院, 助手 (60271954)
IGARASHI Kaoru  School of Dentistry, Tohoku University, Instructor, 歯学部, 助手 (70202851)
SHINODA Hisashi  School of Dentistry, Tohoku University, Professor, 歯学部, 教授 (80014025)
HIRAFUJI Masahiko  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Associate Professor, 薬学部, 助教授 (20142987)
Project Period (FY) 1997 – 1999
KeywordsNitric Oxicide / Periodontal Tissue / Mechanical Stress / Tooth Movement
Research Abstract

Nitric oxide, a mediator of vascular function, neurotransmission, and immune system, has been recently shown to play an important role in adaptive response of bone to mechanical stress/strain. Periodontal tissue receives and adapts to various mechanical stimuli such as masticatory force and orthodontic force. Thus, it is possible that NO is a mediator of adaptive physiological response of periodontal tissue to these mechanical stimuli. In the present study, we tested this possibility both in vitro and in vivo.
1) Effect of cyclic tension force on NO and prostaglandin EィイD22ィエD2 production and mRNA expression of their synthases in human periodontal ligament cells.
Application of cyclic tension force to human periodontal ligament cells induced a rapid and transient increase in NO release in the culture medium. Endothelial isoform of NO synthase (NOS) mRNA expression decreased. Release of prostaglandin EィイD22ィエD2 time-dependently increased by the application of force. Cyclooxygenase-2 mRNA expression increased, whereas cyclooxygenase-1 mRNA expression did not change. These results suggest that NO and PGEィイD22ィエD2 are involved in the adaptive response of periodontal ligament cells to mechanical stress/strain.
2) Effect of topical administration of inhibitors of NO synthase and spontaneous NO donors on orthodontic tooth movement in rats.
Upper first molars of male 8-week-old Wistar rats were moved buccally for 21 days. The topical administration of L-NAME (10 mg/mL), a general inhibitor of NOS activity, caused a significant reduction of tooth movement. On the other hand, L-NIO (5 mg/mL), a selective inhibitor of inducible isoform of NOS, had no effect on tooth movement. There was a tendency toward a decrease in tooth movement in animals treated with NO donors (NOR4 and NOC). These results suggest that NO sythesized by constitutive isoforms of NOS has an important role in orthodontic tooth movement.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 山口希実: "周期的伸展力を加えたヒト歯根膜細胞におけるNOおよびPGE_2の産生と合成酵素(NOS,COX)の遺伝子発現"歯科基礎医学会雑誌. 40. 444 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kotaro Miyoshi: "Tooth movent and changes in periodontal tissue in response to orthodontic force in rats vary depending on the time of day the force is applied"Eur.J.Orthod.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaoru Igarashi: "Diurnal variation of tooth movement in response to orthodontic force in rats"Am.J.Orthod.Dentofac.Orthop.. 114. 8-14 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Hirafuji: "5-Hydroxytryptamine induces transient Ca^<2+> influx through Ni^<2+>-insensitive Ca^<2+> channels in rat vascular smooth muscle cells"Europ.J.Pharmacol.. 380. 163-170 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Hirafuji: "Suppressive effect of serotonin on nitric oxide production by vascular smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats"Jpn.Heart J.. 39. 560 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Hirafuji: "Effect of docosahexaenoic acid on intracellular calcium dynamics in vascular smooth muscle cells from normotensive and genetically hypertensive rats"Res.Commun.Mol.Pathol.Pharmacol.. 102. 29-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Hirafuji: "Progress in Hypertention, Vol.4"VSP BV(Utrecht, The Netherlands). 266 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Hirafuji: "The Biology of Nitric Oxide, Part6"Portland Press(London and Miami). 350 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nozomi Yamaguchi, et al.: "Effect of cyclic tension force on NO and prostaglandin E2 production and mRNA expression of their synthases in human periodontal ligament cells."Jpn. J. Oral Biol.. vol. 40. 444 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kotaro Miyoshi, et al.: "Tooth movement and changes in periodontal tissue in response to orthodontic force in rats vary depending on the time of day the force is applied."Eur. J. Orthod.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaoru Igarashi, et al.: "Diurnal variation of tooth movement in response to orthodontic force in rats."Am. J. Orthod. Dentofac. Orthop. vol. 114. 8-14 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Hirafuji, et al.: "Changes in intracellular CaィイD12+ィエD1 signaling induced by decosahexaenoic acid in vascular smooth muscle cells from a genetically hypertensive rats (SHRSP), In, Progress in Hypertension, Vol. 4."VSP BV. 157-173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Hirafuji, et al.: "5-Hydroxytryptamine induces transient CaィイD12+ィエD1 influx through NiィイD12+ィエD1-isensitive CaィイD12+ィエD1 channels in rat vascular smooth muscle cells."Europ. J. Pharmacol. vol. 380. 163-170 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Hirafuji, et al.: "Nitric Oxide Production by vascular Smooth Muscle Cells From Stroke-prone Spontaneously Hypertensive Rats, In, The Biology of Nitric Oxide, Part 6"Portland Press. 153 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Hirafuji, et al.: "Suppressive effect of serotonin on nitric oxide production by vascular smooth muscle cells isolate from stroke-prone spontaneously hypertensive rats."Jpn. Heart J.. vol. 39. 560 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Hirafuji, et al.: "Effect of docosahexaenoic acid on intracellular calcium dynamics in vascular smooth muscle cells from normotensive and genetically hypertensive rats."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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