1998 Fiscal Year Final Research Report Summary
Development of A Fiber Nano-scope with Molecular Trapping and its Application to the Analysis of Molecular Mechanism of Allergy
| Project/Area Number |
09470495
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MASUJIMA Tsutomu Hiroshima University, Faculty of Medicine Professor, 医学部, 教授 (10136054)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Atsushi Hiroshima University, Faculty of Medicine Research Associate, 医学部, 助手 (30261225)
OZAWA Kouichiro Hiroshima University, Faculty of Medicine Assistant Professor, 医学部, 講師 (10211822)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Molecular Trapping / HPLC resin / MALDI-TOF Mass Spectrometry / Video Nanoscope / Optical Fiber / Cell Dynamism / Brownian Motion |
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| Research Abstract |
1. Development of Molecular Trapping Method and its Application to the Analysis of Allergy
Secreted molecules out of a mast cell model, RBL-2H3, were trapped by ion-exchange resins for HPLC in a single cell revel and its time course of secretion was monitored and analyzed by a fiber nano-scope. One species of the trapped molecules are identified to be histamine by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS). The molecular trapping probe of fiber type with ODS residues was also developed.
2. Development of A Fiber Nano-scope
A video-nanoscope with micro spot lighting with optical fiber light-guide was also developed. The Brownian movement of colloidal gold nano-particles was observed by this video scope and the movement of particleswas analyzed to evaluate the diameters of the particles. The dynamic movement of bound gold-labeled molecules was also observed by this new nano-scope.
3. Molecular Trapping and MALDI-TOF/MS Analysis
Not only the histamine but also the insulin which was secreted out of the beta pancreatic model cells was found to be detected and applied to the time course analysis of the beta pancreatic cell response.
4. Application to Another System
This method was also applied to the analysis of phagocytosis of neutrophils to clarify the sequential dynamism of the cells and clarified many new aspects of its behavior.
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