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1998 Fiscal Year Final Research Report Summary

Mechanism by which Helicobacter pylori infection causes gastric ulcers Mongolian gerbils

Research Project

Project/Area Number 09470508
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

OKABE Susumu  Kyoto Pharmaceutical University, Department of Applied Pharmacology, Professor, 薬学部, 教授 (90012624)

Co-Investigator(Kenkyū-buntansha) AMAGASE Kikuko  Kyoto Pharmaceutical University, Department of Applied Pharmacology, Research As, 薬学部, 助手 (60278447)
TAKAHASHI Satoru  Kyoto Pharmaceutical University, Department of Applied Pharmacology, Associate P, 薬学部, 助教授 (20268098)
Project Period (FY) 1997 – 1998
KeywordsHelicobacter pylori / Mongolian gerbil / gastric ulcer / CINC / neutrophil / gastric acid / gastric mucuc / prostaglandin
Research Abstract

This study was designed to clarify the mechanism of Helicobocter pylori (H.pylori)-induced gastric ulcers in Mongolian gerbils. H.pylori of a VacA- and CagA-positive standard strain (NCTC11637) was once orally inoculated to the animals. H.pylori infection resulted in mucosal inflammation including neutrophil infiltration, associated with cytokine-induced neutrophil chemoattractant (CINC) expression. Thereafter, hemorrhagic lesions occurred in the fundus, followed by generation of ulcers in the same area. Eradication of H.pylori eliminated both CINC expression and gastritis with lesions. Inhibition of acid secretion by omeprazole also improved H.pylori-induced gastric pathology, On the other hand, H.pylori infection impaired mucus synthesis prior to ulceration, and exposure of gastric epithelial cells to H.pylori caused a marked decrease in mucus secretion. Interferon-gamma (IIFNgamma) was induced when gastritis became severe, and exerted a potent inhibitory effect on mucus secretion in the presence of H.pylori. Inhibition of prostaglandin (PG) synthesis by indomethcin accelerated H.pylori-induced gastric ulceration, although the drug did not cause any lesions or ulcers in normal animals. These results suggest that CINC, neutrophil infiltration, gastric acid and IFNgamma serve as deleterious factors in generation of H.pylori-induced gastric ulcers, while gastric mucus and PGs play protective roles in the gastric mucosa.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 毛戸祥博ほか: "砂ネズミにおけるHelicobacter pylori感染胃潰瘍モデルの作製" 実験潰瘍(Ulcer Research). 24. 194-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 毛戸祥博ほか: "Helicobacter pylori感染砂ネズミにおける薬剤の抗潰瘍効果" 実験潰瘍(Ulcer Research). 25. 293-295 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi S.et al.: "Pathological changes in the formation of Helicobacter pylori-induced gastric le-sions in Mongolian gerbils." Dig.Dis.Sci.43. 754-765 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi S.et al.: "Effects of cytokines, without and with Helicobacter phlori components, on mucus secretion by cultured gastric epithelial cells" Dig.Dis.Sci.43. 2301-2308 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keto Y.et al.: "Healing of Helicobacter pylori-induced gastric ulcers in Mongolian gerbils : Com-bined treatment with omeprazole and clarithromycin" Dig.Dis.Sci.44. 257-265 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, E.et al.: "Effects of sucralfate in combination with antimicrobials and an acid pump inhibi-tor on Helicobacter pylori infection in Mongolian gerbils" Ther.Res.19. 3599-3606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Keto, Satoru Takahashi, Susumu Okabe: "Establishment of gastric ulcer model of Helicobacter pylori infection in Mongolian gerbils." Ulcer Research. Vol.24. 194-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihiro Keto, Susumu Okabe: "Effects of drugs on H.pylori-induced gastric ulcers in Mongolian gerbils." Ulcer Research. Vol.25. 293-295 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru Takahashi, Yoshihiro Keto, Hiromi Fujita, Hideaki Muramatsu, Takeshi Nishino, Susumu Okabe: "Pathological changes in the formation of Helicobacter pylori-induced gastric lesions in Mongolian gerbils." Dig.Dis.Sci.Vol.43. 754-765 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru Takahashi, Eiji Nakamura, Susumu Okabe: "Effects of cytokines, without and with Helicobacter pylori components, on mucus secretion by cultured gastric epithelial cells." Dig.Dis.Sci.Vol.43. 2301-2308 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eiji Nakamura, Kana Kitada, Susumu Okabe: "Effects of sucralfate in combination with antimicrobials and an acid pump inhibitor on Helicobacter pylori infection in Mongolian gerbils." Ther.Res.Vol.19. 3599-3606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihiro Keto, Satoru Takahashi, Susumu Okabe: "Healing of Helicobacter pylori-induced gastric ulcers in Mongolian gerbils : Combined treatment with omeprazole and clarithromycin." Dig.Dis.Sci.Vol.44. 257-265 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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