1998 Fiscal Year Final Research Report Summary
Microbial treatment of malodor pollution
| Project/Area Number |
09480134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境保全
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
OHTA Yoshiyuki Fac.of Appl.Biol.Sci., HIROSHIMA UNIVERSITY Prof., 生物生産学部, 教授 (10034464)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Malodor pollution / hydrogen sulfide / malodor / Waste / biofilter |
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| Research Abstract |
Malodor pollution is one of the most serious problems in our country. Especially, the pollution from domestic animal houses is one of the main causes. The solution of the problems requires a large scale of apparatus and a lot of running costs. At the present time the main treatment of malodors is carried out chemically and physically : the malodor components are oxidized with ozone or are treated with activated charcoal etc. Microbial treatment is now realized to be a useful method to treat the malodor. We have found microorganisms capable of growing well and deodorizing malodorous agricultural wastes such as pig feces, domestic garbage etc. In 1998, one strain was isolated which could utilize volatile fatty acids as its sole carbon source. It was identified as Rhodococcus sp. It was immobilized onto ceramic beads for preparation of biofilter in order to capture malodorous n-valeric acid in the exhaust air.
Under suitable conditions, a few hundred ppm of n-valeric acid in the air was completely removed for 20days. This year, a heat stable microorganisms with an ability to deodorize hydrogen sulfide was isolated from the seed culture. This strain was identified as Bacillus sp. The culture conditions for removing of hydrogen sulfide were pH 7-9 and 30-45ﾟC, respectively. The pure culture of the strain was prepared with wheat bran solid medium. The culture was inoculated into chicken feces at 3XLo^6cfu/g of feces. Hydrogen sulfide in the feces was completely removed for 20 hours-cultivation. The cell free extract had also an ability to remove hydrogen sulfide. Now, the biofilter for removing of hydrogen sulfide is under construction.
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