1998 Fiscal Year Final Research Report Summary
Analysis of the mechanism of eukaryotic transcription initiation and its regulation based on the TATA box-binding factor TFIID
| Project/Area Number |
09480159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORIKOSHI Masami The University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助教授 (70242089)
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| Project Period (FY) |
1997 – 1998
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| Keywords | TATA-box binding factor / TFIID / TBP / Transcription regulation / Transcription activation / Chromatin transcription / Transcriptional regulatory factor / Interaction between DNA and transcription factors |
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| Research Abstract |
TATA box-binding factor TFIID plays a crucial role in machanisms of transcription activation. We elucidated the significance of function and structure of TFIID in eukaryotic transcription initiation and its regulation by several methods.
1. Mechanism of the transcriptional activation through the competitive binding of Drosophila TAF_<II>230 and the transcriptional activator VP16
2. Functional analysis of specific interactions of human TAF_<II>100, one of the TFIID subunit
3. Analysis of the involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>22
4. Analysis of restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis
5. Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif
6. Elucidation of novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip60
7. Elucidation of defect in cytokinesis of fission yeast induced by mutation in the WD40 repeat motif of a TFIID subunit
8. Hypothesis of mechanism of how histone acetyltransferases select lysine residues in core histones
9. Isolation and initial characterization of GBF ; a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter
10. Analysis of acetylation of six lysines of a specific class by Tip 60 in core histones in vitro
11. Analysis of TFIIH subunit, through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs
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