1998 Fiscal Year Final Research Report Summary
Study of molecular mechanism of apoptosis using Drosophila melanogaster ectopical expressing ICE
| Project/Area Number |
09480186
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
TSUJIMOTO Yoshihide Osaka University Medical School, Professor, 医学部, 教授 (70132735)
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| Project Period (FY) |
1997 – 1998
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| Keywords | apoptosis / Drosophila / caspase / ICE / programd cell death |
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| Research Abstract |
Apoptosis is an important self-destruction mechanism involved in a variety of biological phenomena including morphogenesis and maintenance of tissue homeostasis. Apoptosis is an important theme not only in biology but also in medical field because dysregulation of apoptosis leads to various diseases such as cancer and neurodegenerative diseases. Although apoptosis is known to be mediated by a unique family of cysteine proteases, called caspase family (originally ICE family), the molecular basis of apoptosis is still largely unknown, particularly how signal transduction downstream of caspases proceeds. To obtain some insights in signal transduction pathway downstream of caspases, we have chosen a genetical system with Drosophila. We have established several lines of Drosophila in which human ICE (or caspase-1) is ectopically expressed, resulting in morphological abnormalities or lethality. Starting with these lines, we have obtained many mutants after random mutagenization with EMS, that bypass the abnormalities. By carrying out genetic mapping, we have identified several apparently novel genes in three chromosomes, that are involved in apoptotic cell death. The information we have obtained from these genetical analyses provides a solid basis from which we are able to molecularly clone these apparently interesting genes.
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[Publications] Shigenaga, A., Funahashi, Y., Kimura, K., Kobayakawa, Y., Kamada, S., Tsujimoto, Y.and Tanimura, T.: "Targeted expression of ced-3 and Ice induces programd cell death in Drosophila." Cell Death & Differ. 4. 71-377 (1977)
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「研究成果報告書概要(欧文)」より
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[Publications] Kamada, S., Washida, M., Hasegawa, J., Kusano, H., Funahashi, Y.and Tsujimoto, Y.: "Involvement of caspase-4(-like) protease in Fas-mediated apoptotic pathway." Oncogene. 15. 285-290 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kagaya, S., Kitanaka, C., Noguchi, K., Mochizuki, T., Sugiyama, A., Asai, A., Yasuhara, N., Eguchi, Y., Tsujimoto, Y.and Kuchino, Y.: "A functional role for death proteases in s-Myc- and c-Myc-mediated apoptosis." Mol.Cell.Biol.17. 6739-6745 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Kamada, S., Kusano, H., Fujita, H., Ohtsu, M., Koya, R.C., Kuzumaki, N.& Tsujimoto, Y.: "A cloning method for caspase substrates that uses the yeast two-hybrid system : Cloning of the anti-apoptotic gene gelsolin." Proc.Natl.Acad.Sci.USA.95. 8532-8537 (1998)
Description
「研究成果報告書概要(欧文)」より
