1999 Fiscal Year Annual Research Report

植物の自他認識システムの分子・細胞生物学的解析

Research Project

Project/Area Number	09480195
Research Institution	Nara Institute of Science and Technology
Principal Investigator	磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)
Co-Investigator(Kenkyū-buntansha)	高山誠司奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教授 (70273836) 蔡晃植奈良先端科学技術大学院大学, バイオサイエンス研究科, 助手 (00263442)
Keywords	自家不和合性 / S-遺伝子 / 花粉因子 / アブラナ / 植物病原菌 / フラジェリン / Pseudomonas avenae / 自他認識
Research Abstract	1.アブラナ科植物の自家不和合性認識反応における花粉因子の探索(磯貝・高山) アブラナ科植物の自家不和合性では、これまで、S遺伝子産物としては柱頭側の因子として、Sレセプターキナーゼ(SRK)とS糖タンパク質(SLG)が明らかとなっていたが、花粉側の因子は、不明であった。本研究では、SLGと相互作用をする花粉抽出物の精製、蛍光ディファレンシャルディスプレーを用いたS遺伝子型で異なる花粉mRNAの探索を通じて、花粉因子の候補分子を見いだし、その分子のS遺伝子間での多様性、SRKなどとのゲノム上での位置関係、花粉や、葯での発現様式を解析し、さらに、この遺伝性の形質転換実験、および、柱頭上での花粉の吸水反応を指標とした生物検定系によって、これが花粉因子であることを確定することが出来た。便宜的にSP11と命名した花粉因子は、システイン8個を含む分子量約70000のタンパク質で、アミノ酸組成はS遺伝子毎に大きく変異していたが、シグナルペプチド部分は高く保存されていた。 2.植物病原菌のフラジェリンと相互作用する因子の探索(蔡) 単子葉植物を宿主とする植物病原菌Pseudomonas avenaeのうち、イネを宿主としない菌株は、細菌を認識して、抵抗性反応を示す。まず、この抵抗反応はある種の過敏感反応であることを細胞生物学的に明らかにした。さらに、この抵抗反応を誘導する細菌側の因子が、鞭毛を構成するフラジェリンであることを、フラジェリンタンパク質の精製及び、フラジェリン欠損菌株の作成によって確証した。また、菌株ごとのフラジェリン分子の差異が、分子の中央部分に集中していることをあきらかにした。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] G.Suzuki: "Highly conserved 5'-flanking regions of two self-incompatibility genes,SLG9 and SPK9.,"Gene. 191. 123-126 (1997)
[Publications] M.Watanabe: "Molecular mechanisms underying self-incompatibility in Brassicacea.,"J.Reprod.Develop.,. 43(suppl). 49-50 (1997)
[Publications] G.Suzuki: "Genomic structure of SLG9 and SRK9 genes in self-incompatible Brassica campestris L."J.Reprod.Develop.,. 43(suppl). 165-166 (1997)
[Publications] G.Suzuki: "Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector"Gene. 199. 133-137 (1997)
[Publications] M.Watanabe: "Molecular characterization of SLG and S-related genes in a self-incompatible Brassica campestres L.var.vellow sarson"Sex.Plant Reprod.,. 10. 332-340 (1997)
[Publications] M.Iwano: "Visualization of a self-incompatibility gene in Brassica camperstris L.by multicolor FISH"Theoret.Appl.Genet.,. 96. 751-757 (1998)
[Publications] Y.Sasaki: "Localization of an SLG protein expressed under the regulation of a tapetum-specefic promoter in anthers of transgenic Brassica napus"Sex.Plant Reprod.,. 11. 245-250 (1998)
[Publications] M.Watanabe: "Sequence comparison of four SLR1 alleles in Brassica campestris (syn.B.rapa)"Sex.Plant Reprod.,. 11. 295-296 (1998)
[Publications] T.Entani: "Centeomeric localization of an S-Rnase gene in petunia hybrida Vilm"Theopr.Appl.Genet.,. 99. 391-397 (1999)
[Publications] M.Watanabe: "Molecular biology of self-incompatibility in Brassica species"Plant Biotechnol.,. 16(4). 263-272 (1999)
[Publications] G.Suzuki: "Genomic oraganization of the S locus : Identification and charasterization of genes in SLG/SRK ragion of S9 faplotype of Brassica campestris (svn.rap).,"Genetics. 153. 391-400 (1999)
[Publications] T.Takasaki: "Inteoduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haploype,but confers no new S haplotype sepcificitv in Brassica rapa L."Plant Mol.Biol.,. 40. 659-668 (1999)
[Publications] F.-S.Che,: "Biochemical and morphological features of rice cell death induced by Psudomonas avenae"Plant Cell Physiol.,. 40(19). 1036-1045 (1999)
[Publications] M.Watanabe: "Two anther-expressed genes downstream downstram of SLG9: Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stge of Brassica campesteis(svn.rapa) L.,"Sex.Plant Reprod.. 12. 127-135 (1999)
[Publications] T.Entani,: "Relationship between polyploydy and pllen self-incompatibility in petunis hybrida Vilm"Biosci.Biotechnol.Biochem.,. 63(11). 1882-1888 (1999)
[Publications] T.Takasaki,: "The S receptor kinase determines self-incompatibility in Brassica stigma"Nature. (in press).
[Publications] S.Takayama: "The pollen determinant of self-incompatibility in Brassica campestris.,"Proc.Natl.Acad.Sci.USA (in press). (in press).
[Publications] M.Iwana: "X-ray microanalysis of Papillar cells and possin grains in the pollination process in Brassica using variable-pressure scanning electron microscope.,"J.Electron Microsc. (in press).
[Publications] S.Takayama: "Isolation and characterization of pollen coat proteins of Brassica cpmpestris that interact with S-locus realted glvcoprotein 1 involved in pollen-stigma adhesion"Proc.Natl Acad.Sci.USA. (in press).

1999 Fiscal Year Annual Research Report

植物の自他認識システムの分子・細胞生物学的解析

Principal Investigator

磯貝 彰 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)

Research Products

[Publications] G.Suzuki: "Highly conserved 5'-flanking regions of two self-incompatibility genes,SLG9 and SPK9.,"Gene. 191. 123-126 (1997)

[Publications] M.Watanabe: "Molecular mechanisms underying self-incompatibility in Brassicacea.,"J.Reprod.Develop.,. 43(suppl). 49-50 (1997)

[Publications] G.Suzuki: "Genomic structure of SLG9 and SRK9 genes in self-incompatible Brassica campestris L."J.Reprod.Develop.,. 43(suppl). 165-166 (1997)

[Publications] G.Suzuki: "Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector"Gene. 199. 133-137 (1997)

[Publications] M.Watanabe: "Molecular characterization of SLG and S-related genes in a self-incompatible Brassica campestres L.var.vellow sarson"Sex.Plant Reprod.,. 10. 332-340 (1997)

[Publications] M.Iwano: "Visualization of a self-incompatibility gene in Brassica camperstris L.by multicolor FISH"Theoret.Appl.Genet.,. 96. 751-757 (1998)

[Publications] Y.Sasaki: "Localization of an SLG protein expressed under the regulation of a tapetum-specefic promoter in anthers of transgenic Brassica napus"Sex.Plant Reprod.,. 11. 245-250 (1998)

[Publications] M.Watanabe: "Sequence comparison of four SLR1 alleles in Brassica campestris (syn.B.rapa)"Sex.Plant Reprod.,. 11. 295-296 (1998)

[Publications] T.Entani: "Centeomeric localization of an S-Rnase gene in petunia hybrida Vilm"Theopr.Appl.Genet.,. 99. 391-397 (1999)

[Publications] M.Watanabe: "Molecular biology of self-incompatibility in Brassica species"Plant Biotechnol.,. 16(4). 263-272 (1999)

[Publications] G.Suzuki: "Genomic oraganization of the S locus : Identification and charasterization of genes in SLG/SRK ragion of S9 faplotype of Brassica campestris (svn.rap).,"Genetics. 153. 391-400 (1999)

[Publications] T.Takasaki: "Inteoduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haploype,but confers no new S haplotype sepcificitv in Brassica rapa L."Plant Mol.Biol.,. 40. 659-668 (1999)

[Publications] F.-S.Che,: "Biochemical and morphological features of rice cell death induced by Psudomonas avenae"Plant Cell Physiol.,. 40(19). 1036-1045 (1999)

[Publications] M.Watanabe: "Two anther-expressed genes downstream downstram of SLG9: Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stge of Brassica campesteis(svn.rapa) L.,"Sex.Plant Reprod.. 12. 127-135 (1999)

[Publications] T.Entani,: "Relationship between polyploydy and pllen self-incompatibility in petunis hybrida Vilm"Biosci.Biotechnol.Biochem.,. 63(11). 1882-1888 (1999)

[Publications] T.Takasaki,: "The S receptor kinase determines self-incompatibility in Brassica stigma"Nature. (in press).

[Publications] S.Takayama: "The pollen determinant of self-incompatibility in Brassica campestris.,"Proc.Natl.Acad.Sci.USA (in press). (in press).

[Publications] M.Iwana: "X-ray microanalysis of Papillar cells and possin grains in the pollination process in Brassica using variable-pressure scanning electron microscope.,"J.Electron Microsc. (in press).

[Publications] S.Takayama: "Isolation and characterization of pollen coat proteins of Brassica cpmpestris that interact with S-locus realted glvcoprotein 1 involved in pollen-stigma adhesion"Proc.Natl Acad.Sci.USA. (in press).

磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)