• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

The Mechanism for Formation and Maintenance of Synapses in the Prefrontal Cortex by Biogenic Amines

Research Project

Project/Area Number 09480210
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionUniversity of Tsukuba

Principal Investigator

OKADO Nobuo  Univ.Tsukuba, Inst Basic Med Sci, Professor, 基礎医学系, 教授 (50060140)

Co-Investigator(Kenkyū-buntansha) HAMADA Shunn  Univ.Tsukuba, Inst Basic Med Sci, Assistant Professor, 基礎医学系, 講師 (60282349)
SHIGA Takashi  Univ.Tsukuba, Inst Basic Med Sci, Associate Professor, 基礎医学系, 助教授 (50178860)
Project Period (FY) 1997 – 1998
KeywordsPrefrontal cortex / biogenic amines / synapse / plasticity / SEROTONIN / RECEPTOR
Research Abstract

5-HT2A receptor has been shown to mediate the function of 5-HT for formation and maintenance of synapses in the developing and mature brain. We raised specific antibodies against receptor protein of 5-HT2A receptor. By using the antibodies distribution pattern of 5-HT2A receptor was examined in the cerebral cortex of the rat. Immunoreaction of 5-HT2A receptor was located diffusely in the cerebral cortex. Virtually all the neurons in the cerebral cortex had the immunoreactivity, and specially somal profiles and apical dendrites had strong immunoreaction. Astrocyte identified by using an antibody GF AP showed a strong immunoreaction of 5-HT2A receptors. Although discrepancies in terms of exsistence of 5-HT2A receptors in the cerebellum between conventional morphological and functional studies, our immunohistochemical study has shown a strong immunoreaction in the Purkinje cells. By using an immuno- electron microscopical technique 5-HT2A receptor was located just beneath the postsynaptic membrane thicknings of asymmetrical synapses, possibly glutamatergic ones. To clarify regulatory mechanism for glutamatergic receptors by 5-HT changes in receptor bindings of glutamate were examined in the cerebral cortex 6 week-old rat after 5-HT depletion for a week. AMPA receptor, specially GluR2 was upregulated. 5-HT appears to regulate synaptic plasticity into two way : regulating the number of synapses and Ca permeability by changing composition of Quill receptor suntypes.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Hayashi,Nagaoka,Yamada,Ichitani,Miake,Okada: "Materna lestrass induces syngtic loss and developmental disabilities of offspring" Ins.J.Develop.Neuroscience. 16. 209-216 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maeshima,Shutoh,Hamada,Senzaki,Itoh: "Serotonin 2A receptor-like cimmuoractirity an rat cerebeller Purkinge cells" Neurosci.Lett.252. 72-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maeshima,Ito,Hamada,Senzaki,Shutoh,Okada: "The cellularlocalization of 5-HT2A receptors in the Spinal Cord and Spinal ganglia of the adult rat" Brain Res.797. 118-124 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamada,Senzaki,Hamaguchi,Hamada,Tabuchi: "Localization of 5-HT2A receptor in the rat cerabrel cortex and olfactory bulb reveled by immunohisteclemustry" Mol. Brain Res. 54. 199-211 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsukawa,Ogawa,Nakadate,Maeshima,Ichitani,Kawai,Okada: "Serotonin and acetylchdine are crucialto inacntain hippocamped synopsse and memory seyuisition in rats." Neurosci.Lett. 230. 13-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi, A., Nagaoka, M., Yamada, K., Ichitani, Y., Miake, Y., and Okado, N.: "Maternal stress induces synaptic loss and developmental disabilities of offspring." Int.J.Dev.Neuroscience. 16. 209-216 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maeshima, T., Shutoh, F., Hamada, S., Senzaki, K., Hamaguchi-Hamada, K., Ito, R., and Okado, N.: "Serotonin2A receptor-like immunoreactivity in rat cerebellar Purkinje cells." Neurosci.Lett.252. 72-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maeshima, T., Ito, R., Hamada, S., Senzaki, K., Hamaguchi-Hamada, K., Shutoh, F., and Okado, N.: "The cellular localization of 5-HT2A receptors in the spinal cord and spinal ganglia of the adult rat." Brain Res.797. 118-124 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamada, S., Senzaki, K., Hamaguchi-Hamada, K., Tabuchi, K., Yamamoto, H., Yamamoto, T., Yoshikawa, S., Okano, H., and Okado, N.: "Localization of 5-HT2A receptor in rat cerebral cortex and olfactory system revealed by immunohistochemistry using two antibodies raised in rabbit and chicken." Mol.Brain Res.54. 199-211 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsukawa M,Ogawa M,Nakadate K,Maeshima T,Ichitani Y,Kawai N,Okado N: "Serotonin and acetylcholine are crucial to maintain hippocampal synapsesand memory acquisition in rats." Neurosci Lett. 230. 13-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi