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1999 Fiscal Year Final Research Report Summary

Local neural circuit in the cerebral cortex

Research Project

Project/Area Number 09480211
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KANEKO Takeshi  Gradudate School of Medicine, KYOTO UNIVERSITY Professor, 医学研究科, 教授 (90177519)

Project Period (FY) 1997 – 1999
KeywordsCerebral Cortex / Local Neural Circuit / Pyramidal cell / Non-pyramidal cell / Transgenic mouse / GABAergic neuron / Glutamtergic neuron / Brain slice
Research Abstract

1. Connections of layer III pyramidal neurons to corticospinal of layer V and corticothalamic neurons of layer VI in the rat primary motor cortex were examined in brain slices by combining intracellular staining with Golgi-like retrograde labeling of corticofugal neurons. Forty layer III pyramidal neurons stained intracellularly were of the regular-spiking type, showed immunoreactivity for glutaminase, and emitted axon collaterals arborizing locally in layers II/III and/or V. Nine of them were reconstructed for morphological analysis;15.2% or 3.8% of varicosities of axon collaterals of the reconstructed neurons were apposed to dendrites of corticospinal or corticothalamic neurons, respectively. By con-focal laser-scanning and electron microscopy, some of these appositions were revealed to make synapses. These findings suggest that corticospinal neurons receive information from the superficial cortical layers four times more frequently than corticothalamic neurons.
The connections were f … More urther examined by intracellular recording of excitatory postsynaptic potential(EPSP)that were evoked in layer V and layer VI pyramidal neurons by stimulation of layer II/III. EPSPs evoked in layer V pyramidal neurons showed short and constant onset latencies, suggesting their monosynaptic nature. In contrast, most EPSPs evoked in layer VI pyramidal had long onset latencies, showed double-shock facilitation of onset latency, and were largely suppressed by an NMDA receptor blocker, implying that they were polysynaptic.
The results suggest that information from the superficial cortical layers is transferred directly and efficiently to corticospinal neurons in layer V and there by exerts an important influence on cortical motor output. Corticothalamic neurons are, in contrast, considered relatively independent of, or indirectly related to, information processing of the superficial layers.
2. We have succeeded in retrograde Golgi-stain-like visualization of cortical pyramidal cells by infection of adenovirus vector which contain the expression system of green fluorescent protein(GFP)attached with membrane-targetting signal.
3. We are now trying the cloning of promoter regions of calretinin and parvalbumin, which are the marker for the subgroups of cortical GABAergic interneurons. The interneurons will be labeled in a Golgi-stain manner by specific expression of membrane-targetted GFP in transgenic mice using the promoter regions. Less

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kaneko,Takeshi: "Predominant information transfer from layer III pyramidal neurons to corticospinal neurons"The Journal of Comparative Neurology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taki,Kousuke: "A group of cortical interneurons expressing μ-opioid receptor-like immunoreactivity : A double immunofluorscence study in the rat cerebral cortex"Neuroscience. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kang,Yungnam: "Transsynaptically induced bursts in regular spiking non-pyramidal cells in deep layers of the cat motor cortex"Cerebral Cortex. 9. 77-89 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko,Takeshi: "Characterization of neocortical non-pyramidal neurons expressing preprotachykinins A and B : A double immunofluorescence study in the rat"Neuroscience. 86. 765-781 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee,Teffy: "Preprodynnorphin-, preprotachykinin-expressing neurons in the rat neostriatum : An analysis by immunocytochemistry and retrograde tracing"The Journal of Comparative Neurology. 386. 229-244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee,Teffy: "Collateral projections from striatonigral neurons to substance P receptor-expressing intrinsic neurons in the striatum of the rat"The Journal of Comparative Neurology. 388. 250-264 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li Y-Q, Takada M, Kaneko T, and Mizuno N: "Distribution of GABAergic and glycinergic premotor neurons projecting to the facial and hypoglossal nuclei in the rat"The Journal of Coomparative Neurology. vol. 378. 283-294 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J-L, Kaneko T, Shigemoto R, Mizuno: "Distribution of trigeminohypothalamic and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity in the rat"The Jouranal of Comparative Neurology. vol. 378. 508-521 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gong L-W, Ding Y-Q, Wang D, Zheng H-X, Qin B-Z, Li J-S, Kaneko T, and Mizuno N: "GABAergic synapses on μ-opioid receptor-expressing neurons in the superficial dorsal horn : an electron microscopic study in the cat spinal cord."Neuroscience Letters. vol. 227. 33-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J-L, Kaneko T, Nomura S, Li Y-Q, and Mizuno N: "Association of serotonin-like immunoreactive axons with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat"The Journal of Comparative Neurology. vol. 384. 127-141 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Caria MA, Kaneko T, Kimura A, and Asanuma H: "Functional organization of the projection from area 2 to area-4γ in the cat"Journal of Neurophysiology. vol. 77. 3107-3114 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen J, Koyama N, Kaneko T, and Mizuno N: "The locus coeruleus of the Japanese monkey (Macaca fuscata) does not express μ-opioid receptor-like immunoreactivity"Brain Research. vol. 755 (no. 2). 326-330 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee T, Kaneko T, Taki K, and Mizuno N: "Preprodynnorphin-, preproenkephalin-, and preprotachykinin-expressing neurons in the rat neostriatum : An analysis by immunocytochemistry and retrograde tracing"The Journal of Comparative Neurology. vol. 386 (no. 2). 229-244 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee T, Kaneko T, Shigemoto R and Mizuno N: "Collateral projections from striatonigral neurons to substance P receptor-expressing intrinsic neurons in the striatum of the rat"The Journal of Comparative Neurology. vol. 388 (no. 2). 250-264 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko T, Murashima M, Lee T and Mizuno N: "Characterization of neocortical non-pyramidal neurons expressing preprotachykinins A and B : A double immunofluorescence study in the rat"Neuroscience. vol. 86 (no. 3). 765-781 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J-L, Ding Y-Q, Li J-S, Nomura S, Kaneko T and Mizuno N: "Immunocytochemical localization of μ-opioid receptor in primary afferent neurons containing substance P or calcitonin gene-related peptide. A light and electron microscopic study in the rat"Brain Research. vol. 794. 347-352 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J-L, Kaneko T, Nomura S, and Mizuno N: "Projections from the caudal spinal trigeminal nucleus to commisural interneurons in the supratrigeminal region : an electron microscope study in the rat"Neuroscience Letters. vol. 254. 57-60 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ino T, Kaneko T, and Mizuno N: "Direct projections from the entorhinal cortical layers to the dentate gyrus, hippocampus, and subicular complex in the cat"Neuroscience research. vol. 32. 241-265 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang Y, Endo K, Araki T and Kaneko T: "Transsynaptically induced bursts in regular spiking non-pyramidal cells in deep layers of hte cat motor cortex"Cerebral Cortex. vol. 9. 77-89 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura K., Kankeo T., Yamashita Y., Hasegawa H., Katoh H., Ichikawa A., and Negishi M.: "Immunocytochemical localization of prostaglandin EP3 receptor in the rat hypothalamus"Neuroscience Letters. vol. 260. 117-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J-L, Li Y-Q, Kaneko T and Mizuno N: "Preprodynorphin-like immunoreactivity in medullary dorsal horn neurons projecting to the thalamic regions in the rat"Neuroscience Letters. vol. 264. 13-16 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ding Y. -Q., Zheng H. -X., Wang D. -S., Xu J. -Q., Gong L. -W., Lu Y., Qin B. -Z., Shi J., Li H. -L., Li J. -S., Shigemoto R., Kaneko T., Mizuno N.: "The distribution of substance P receptor (NK1) -like immunoreactive neurons in the newborn and adult human spinal cord"Neuroscience Letters. vol. 266. 133-136 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li Y. -Q., Li H., Kankeo T., and Mizuno N: "Substantia gelatinosa neurons in the medullary dorsal horn : an intracellular labeling study in the rat"The Journal of Comparative Neurology. vol. 411. 399-412 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li Y. -Q., Li H., Kankeo T., and Mizuno N: "Local circuit neurons showing calbindin D28k-immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat. An immunocytochemical study combined with intracellular staining in slice preparation"Brain Research. vol. 840. 179-183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minoguchi S., Ikeda T., Itohara S., Kaneko T., Okaichi H., and Honjo T: "Studies on thecell-type specific expression of RBP-L, a RBP-J family member, by replacement insersion of b-galactosidase"J. Biochem. vol. 126. 738-747 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J. -L., Li Y. -Q., Li J. -S., Kaneko T., and Mizuno N.: "Calcium-binding protein-immunoreactive projection neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat"Neuorscience Research. vol. 35. 225-240 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ino T, Kaneko T, and Mizuno N: "Intrinsic and commisural connections within the entorhinal cortex. An anterograde and retrograde tract-tracing study in the cat"Neuroscience Research. vol. 36. 45-60 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J. -L., Wang D., Kaneko T., Shigemoto R., Nomura S., and Mizuno N: "Relationship between neurokinin-1 receptor and substance P in the striatum : light and electron microscopic immunohistochemical study in the rat"The Journal of Comparative Neurology. vol. 418. 156-163 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taki K, Kaneko T, and Mizuno N: "A group of cortical interneurons expressing μ-opioid receptor-like immunoreactivity : A double immunofluorescence study in the rat cerebral cortex"Neurosicience. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko T., Cho R. -H., Li Y. -Q., Nomura S., and Mizuno N: "Predominant information transfer from layer III pyramidal neurons to corticospinal neurons"The Journal of Comparative Neurology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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