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1998 Fiscal Year Final Research Report Summary

Effects of perinatal steroid hormonal changes on the cytoarchitecture of brain

Research Project

Project/Area Number 09480214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KAWATA Mitsuhiro  Kyoto Pref.Univ.of Medicine, Professor, 医学部, 教授 (60112512)

Co-Investigator(Kenkyū-buntansha) MORITA Noryuki  Kyoto Pref.Univ.of Medicine, Research Associate, 医学部, 助手 (50239662)
NISHI Mayumi  Kyoto Pref.Univ.of Medicine, Assistant Professor, 医学部, 講師 (40295639)
OZAWA Hitoshi  Kyoto Pref.Univ.of Medicine, Associate Professor, 医学部, 助教授 (60169290)
Project Period (FY) 1997 – 1998
Keywordssex hormone / estrogen receptor / postnatal development / apoptosis / over expression / preoptic area / sexual dimorphism / GFP
Research Abstract

To elucidate the mechanism of the formation of sexual dimorphism, we examined the number of apoptotic cells in the medial preoptic nucleus in male and female rats of various developing stages. Many apoptotic cells in the postnatal day 1 and 4 were found in the preoptic periventricular nucleus and its number was significantly larger in male than in female. The number of apoptotic cells in the medial preoptic nucleus was increased in the postnatal day 4 and 7 and significantly larger in female than in male. It was found that the distribution of apoptotic cells depends on the sexuality, the location of the area and postnatal period, indicating that the apoptosis contributes the formation of sexual dimorphism in the medial preoptic area. The medial preoptic area contains highest number of estrogen receptors. Therefore we expanded our experiments to investigate the subcellular localization of estrogen receptor in cultured cells, in which estrogen receptor linked to GFP was over expressed. With or without ligand, the subcellular localization of estrogen receptor was localized in the nucleus, not in the cytoplasm.
It was concluded that the sexual dimorphism in the brain was due, at least in part, to the apotosis of the neurons in the specific areas and these apoptosis was strongly linked to the gene expression of steroid hormone receptor.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ozawa H.,Ito T,Ochiai I,Kawata M: "Cellular localization and distribution of glucocorticoid receptor (GR) immunoreactivity and the expression of GR mRNA in the rat pituitary gland : A combined double immunohistochemistry and in situ hybridization histochemical analysis" Cell Tissue Res. 295. 207-214 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang Y., Ozawa H., Lu H., Hayashi S., Nihonyanagi K., Kawata M.: "Immunocytochemical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion,with special reference to estrogen and its receptor" Brain Res.791. 35-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawata M., Yuri K., Ozawa H., Nishi M, Ito T, Hu Z., Lu H., Yoshida M.: "Steroid hormones and their receptors in the brain" J.Steroid Biochem. Molec. Biol.65. 273-280 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hu Z ., Ito T., Yuri K., Chang X., Ozawa H., Kawata M.: "In vivo time course of morphological changes and DNA degradation during the degeneration of castration-induce apoptotic cells" Cell Tissue Res.294. 153-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lu H., Yuri K., Ito T., Yoshimoto K., Kawata M.: "The effects of oestrogen and progesterone on serotonin and its metabolite in lateral septum,medial preoptic area and ventromedial hypothalamic nucleus of female rats" J.Neuroendocrinology. 10. 919-926 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hu Z., Yuri K., Ozawa H., Lu H., Kawata M.: "The in vivo time course for elimination of adrenalectomy-induced apoptotic profiles from the granu〓〓〓〓ell layer of the rat" J.Neurosci.17. 3981-3989 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河田光博,伊藤隆男,森田規之,西真弓: "組織細胞化学,非放射性標識RNAプローブを用いるin situハイブリダイゼーション法" 学際企画,東京, 121-127 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森田規之,伊藤隆男,河田光博: "脱アイソトープ実践プレトコール,カラーin situハイブリダイゼーション" 秀潤社,東京, 269-299 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozawa H., Ito T, Ochiai I, Kawata M.: "Cellular localization and distribution of glucocorticoid receptor (GR) immunoreactivity and the expression of GR mRNA in the rat pituitary gland : A combined double immunohistochemistry and in situ hybridization histochemical analysis" Cell Tissue Res.295. 207-214 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yang Y., Ozawa H., Lu H., Hayashi S., Nihonyanagi K., Kawata M.: "immunocytochemical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion, with special reference to estrogen and its receptor" Brain Res.791. 35-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawata M., Yuri K., Ozawa H., Nishi M., Ito T, Hu Z., Lu H., Yoshida M.: "Steroid hormones and their receptors in the brain" J.Steroid Biochem.Molec.Biol.65. 273-280 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hu Z., Ito T., Yuri K., Chang X., Ozawa H., Kawata M.: "In vivo time course of morphological changes and DNA degradation during the degeneration of castration-induce apoptotic cells" Cell Tissure Res.294. 153-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lu H., Yuri K., Ito T., Yoshimoto K., Kawata M.: "The effects of oestrogen and progesterone on serotonin and its metabolite in lateral septum, medial preoptic area and ventromedial hypothalamic nucleus of female rats" J.Neuroendocrinology. 10. 919-926 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hu Z., Yuri K., Ozawa H., Lu H., Kawata M.: "The in vivo time course for elimination of adrenalectomy-induced apoptotic profiles from the granule cell layer of the rat" J.Neurosci.17. 3981-3989 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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