1999 Fiscal Year Final Research Report Summary
Identification of novel microglial genes up-regulated during neuronal apoptosis
| Project/Area Number |
09480217
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KOIKE Tatsuro Hokkaido Univ., Grad. School of Sci., Professor, 大学院・理学研究科, 教授 (80128131)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Shuuitsu Hokkaido Univ., Grad. School of Sci., Instructor, 大学院・理学研究科, 助手 (90202431)
WATANABE Masahiko Hokkaido Univ., Grad. School of Med., Professor, 大学院・医学研究科, 教授 (70210945)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Microglia / Apoptosis / Cerebellum / Granule neuron / lysosome |
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| Research Abstract |
To search for genes up-regulated during neuronal cell death, we have employed cerebellar cell cultures, in which glial cells respond to degeneration and cell death of granule neurons that begins to occur at 4 DIV. Differential hybridization was performed with subtracted cDNA probes and a cDNA library from 5 DIV. One of the genes up-regulated during cell death is a novel gene, mrf-1, in microglia. Another gene is highly homologous to the mouse genes with hematopoietic cell origin which may be involved in microglial activation associated with enhanced functions of lysosomes.
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