2001 Fiscal Year Final Research Report Summary
Identification of novel microglial genes up-regulated during neuronal apoptosis
| Project/Area Number |
09480217
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KOIKE Tatsuro Hokkaido Univ., Grad. School of Sci., Professor, 大学院・理学研究科, 教授 (80128131)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Shuuitsu Hokkaido Univ., Grad. School of Sci., Instructor, 大学院・理学研究科, 助手 (90202431)
WATANABE Masahiko Hokkaido Univ., Grad. School of Med., Professor, 大学院・医学研究科, 教授 (70210945)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Microglia / Apoptosis / Cerebellum / Granule neuron / lysosome |
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| Research Abstract |
To searach for genes up-regulated during neuronal cell death, we have employed cerebellar cell cultures, in which glial cells respond to degeneration and cell death of granule neurons that begins to occur at 4DIV. Differential hybridization was performed with subtracted cDNA probes and a cDNA library from 5DIV. One of the genes up-regulated during cell death is a novel gene, mrf-1, in microglia. Another gene is highly homologous to the mouse genes with hematopoietic cell origin which may be involved in microglial activation associated with enhanced functions of lysosomes
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