1999 Fiscal Year Final Research Report Summary
Molecular mechanism for synaptic plasticity mediated by postsynaptic density
| Project/Area Number |
09480222
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Shinshu University |
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Principal Investigator |
SUZUKI Tatsuo Shinshu University School of Medicine, Professor, 医学部, 教授 (80162965)
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| Co-Investigator(Kenkyū-buntansha) |
OKANO Akira Shinshu University School of Medicine, Research Associate, 医学部, 助手 (30020803)
TAKAGI Hiroshi Shinshu University School of Medicine, Associate Professor, 医学部, 講師 (80247220)
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| Project Period (FY) |
1997 – 1999
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| Keywords | PSD / postsynaptic / synaptic plasticity / cloning |
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| Research Abstract |
1. We performed expression cloning of cDNAs using antibody reacting to postsynaptic density (PSD) proteins. We cloned known clones such as those encoding fodrin, plectin and neurofilament, as well as unknown clones. We further proceeded the cloning of unknown clones and isolated a cDNA encoding a synGAP isoform, which we termed as synGAPβ. Furthermore, we identified 7 synGAPmRNAs (splice variants) and 5 synGAP protein isoforms. The synGAPβ protein was found to be specifically localized in the PSD fraction isolated form the rat brain.
2. We have isolated another novel gene from a rat cDNA library. The cDNA encoded 3960 bases and contained 2903 base ORF. The speculated amino acid sequence had homology to talin and Sla2 in the carboxyl terminal half region, and to Huntingtin-interacting protein (HIP) in the whole region. Northern blotting showed 4.2 kb band and distribution in the brain and the testis. The protein was detected by Western blotting in the spleen as well as the brain and the testis, and synaptosome and the synaptic plasma membrane fractions, among subcellular fractions prepared from the rat brain. The distribution of the protein resembled that of Huntingtin: These results suggested roles for synaptic function and those related to Huntingtin in the brain.
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