1999 Fiscal Year Final Research Report Summary
Functional studies of presynaptic proteins in neurotransmitter release
| Project/Area Number |
09480225
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
MOCHIDA Sumiko Tokyo Medical University, Physiology, Associate professor, 医学部, 助教授 (30096341)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Masami Mitsubishi kasei Life Science Institute, Project Leader, 脳神経科学部, プロジェクトリーダー
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| Project Period (FY) |
1997 – 1999
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| Keywords | synaptic vesicles / neurotransmitter / exocytosis / prosynaptic proteins / CaィイD12+ィエD1-binding proteins / CaィイD12+ィエD1 channels |
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| Research Abstract |
To examine roles for presynaptic protein-protein interactions in neurotransmitter release, synapses formed between rat superior cervical ganglion neurons in culture were employed. The synaptic protein interaction site of several presynaptic proteins were synthesized and introduced into the presynaptic neurons through a glass patch pipette, while measuring synaptic transmission evoked by presynaptic action potentials. The change in postsynaptic responses, we speculated the function of the proteins as follows.
1)CaィイD12+ィエD1-binding protein
(A)synaptotagmin : trigger of synaptic vesicle fusion, promotion of endocytosis.
(B)Doc2α-Munc 13 interacion : synaptic vesicle translocation to the release site.
2)SNARE-associating proteins
(A)N-type CaィイD12+ィエD1 channels : involved in synchronous transmitter release, voltage-dependent enhancement of transmitter release.
(B)P/Q-type CaィイD12+ィエD1 channels : involved in neurotransmitter release.
cSnapin : modulation of synaptotagmin-SNARE complex interaction.
(D)Syntaphilin : control of SNARE complex assembly by syntaxin-1 clamping.
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[Publications] Lao,G., Scjeiss,V., Gerwin,C.M., Su,Q., Mochida,S., Rettig,J., Sheng,Z.-H.: "Syntaphilin : a syntaxin-1 clamp that controls SNARE assembly."Neuron. 25(1). 191-201 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mikoshiba, K., Fukuda, M., Niinobe, M., Mochida, S., Sugimori, M., Llinas, R.,: "Role of inositol polyphosphate in exocytosis."In The adrenal Chromaffin Cell. KANNO,T., NAKAZATO,Y., KUMAKURA,K, Eds. Hokkaido University Press.. 213-220 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Mochida,S., Kuba,K.: "Exocytosis and Modulation : Introductory Review."In Slow Synaptic : Responses and Modulation. KUBA,K., HIGASHIDA,H., BROWN,D.A., YOSHIOKA,T., Eds. Springer-Verlag, Tokyo.. 212-234 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Mochida,S.: "Studies of neurotransmitter release at cholinergic synapses formed in culture"In Slow Synaptic : Responses and Modulation KUBA,K., HIGASHIDA,H., BROWN,D.A., YOSHIOKA,T., Eds. Springer-Verlag, Tokyo.. 235-246 (1999)
Description
「研究成果報告書概要(欧文)」より
