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1999 Fiscal Year Final Research Report Summary

DEVELOPMENT OF AN ANIMAL MODEL FOR HEMOLYTIC UREMIA SYNDROME (HUS) WITH NONHUMAN PRIMATES

Research Project

Project/Area Number 09480248
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

YOSHIKAWA Yasuhiro  TOKYO UNIVERSITY GRAUATE SCHOOL OF ACRICULTURE AND LEFE SCIENCES, PROFESSOR, 大学院・農学生命科学研究科, 教授 (80109975)

Co-Investigator(Kenkyū-buntansha) SAKAKIBARA Ippei  IBID, SENIER RESEARCHER, 筑波霊長類センター, 主任研究官
TERAO Keiji  NATIONAL INSTITUTE OF INFECTIOUS DISEASES, TSUKUBA PRIMATE CENTER, CHIEF RESEARCHER, 筑波霊長類センター, 室長(研究職)
KAWAMURA Seiji  IBID, ASSISTANT PROFESSOR, 大学院・農学生命科学研究科, 助教授 (10161366)
Project Period (FY) 1997 – 1999
KeywordsKEY MORDS / cynomolgus monkey / African green monkey / Vero toxins / O-157 / VT1 / VT2 / HUS model / ceramid trihexose
Research Abstract

To develop an animal model for hemolytic uremia syndrome (HUS) using non-human primates, Cercopithecine monkeys such as African green monkeys (Cercopithecus aetiopus) and cynomolgus monkeys (Macaca fascicularis) were used in this experiment. The existence of a glycolipid receptor (ceramid trihexose ; CTH) for Vero toxins (VT1, VT2) was investigated by a biochemical method with thin layer chromatography. As a result, the receptors were more abundantly expressed in a cynomolgus monkey than an African green monkey and they localized mainly in the kidneys, spleen, bone marrow and lungs. Immunohistological studies by the use of a monoclonal antibody recognizing the CTH revealed that it was expressed densely in the mucosal epithelial cells of the small intestine, glomerulus and renal tubules in the kidneys and vascular endothelial cells in the medulla of the spleen and small blood vessels in the whole body. In the in vivo study, 24〜48hrs after Vero toxins (VT1 or VT2) injection, cynomolgus monkeys became moribund and they showed intestinal hemorrhage with thoracic fluid or ascite. Obvious thrombocytepenia, deterioration of a coagulation function and anomalies of the blood biochemical values were demonstrated. Histological examinations exhibited necrosis and severe hemorrhage in the intestinal mucosa, hemorrhage in the adrenal cortex, edematous change in the lungs, vacuolar degeneration of the vascular endothelial cells and degeneration of glomerulus and renal tubules in the kidneys, which are similar to clinicopathological lesions observed in human cases.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 吉川泰弘: "動物由来感染症と検疫"臨床と微生物. 26. 279-286 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川泰弘: "サル由来ウイルス感染症"化学療法の領域. 15. 27-33 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] 吉川泰弘: "学校飼育動物と人獣共通感染症"MVM. 10. 59-63 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi,R. et al.: "Opportunistic pneumocystis carinii in infection in red-bellied tamarins (Saguinus labiatus)"Exp.Anim.. 48. 55-57 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] Akari,F. et al.: "Effect of SIVmac infection on peripheral blood CD4CD8 Tlymphocytes in cynomolgus macaques"Clinic.Immunol.. 91. 321-329 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] Liang,JH. et al.: "Localization of immunoreactive testosterone and 3β-hydroxy steroid dehydrogenase isomerase"J.Med.Primatol.. 28. 62-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉川泰弘: "エボラ出血熱 in 獣医感染症カラーアトラス"文永堂出版. 108-109 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] 吉川泰弘: "Bウイルス感染症 in エマージングディシーズ"近代出版. 265-270 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] Otani, I., Mori, K., Sata, T., Terao, K., Doi, K., Akari, F., Yoshikawa, Y.: "Accumulation of Mac387macrophages in paracortical areas of lymph nodes in rhesus monkeys acutely infected with SIV"Micro.Infect.. 1. 977-985 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Fujii Y., Akari, F., Machida, M., Kojima, E., Murakami, K., Yoshikawa, Y.: "Effective delivery of a liopophilic 6-Cl-ddG into rat lymphoid tissues."Exp.Anim. 48. 241-146 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, K., Yanai, H., Koirasa, Ohara, N., Teramoto, N., Oka, T., Yoshino, T., Takahashi, K., Miyamoto, K., Fujumoto, K., Yoshikawa, Y., Akagi, T.: "Cyno-EBV induced rabbit malignant lymphomas and thier tumor cell lines frequently show specific chromosomal abnormlities"Lab.Invest.. 79. 823-835 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, R., Sakakibara, I., Furuta, T., Kikuchi, T., Yoshikawa, Y.: "Opportunistic pneumocystis carinii infection in red-bellied tamarins (Saguinus labiatus)."Exp.Anim.. 48. 55-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akari, F., Nam, KH., Mori, K., Otani, I., Shibata, I., Adachi, A., Terao, K., Yoshikawa, Y: "Effect of SIVmac infection on peripheral blood CD4CD8 Tlymphocytes in cynomolgus macaques."Clinic.Immunol.. 91. 321-329 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Odagiri, K., Hamano, M., Yoshikawa, Y.: "Egg-eating behavior in laboratory squirrel monkeys (Saimiri scuireus)."J.Vet.Med.Sci.. 61. 595-601 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Liang, JH., Sankai, T., Yoshida, T., Cho, F., Yoshikawa, Y: "Localization of immunoreactive testosterone and 3β-hydroxysteroid dehaydrogenase isomerase in cynomolgus monkey testes during postnatal development."J.Med.Primatol.. 28. 62-66 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, H., Katayama, K., Ikawa, A., Miyawaki, K., Shinozuka, J., Uetsuka, K., Nakamura, S., Kimura, N., Yoshikawa, Y., Doi, K.: "Cerebral amyloid angiopathy in an aged spotted woodpecker."Neurobiol.Aging. 20. 53-56 (1999)

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Published: 2002-03-26  

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