1998 Fiscal Year Final Research Report Summary
Construction of gene tagging system by means of the medaka fish transposable element Tol-2
| Project/Area Number |
09554053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
遺伝
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| Research Institution | Nagoya University |
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Principal Investigator |
HORI Hiroshi Graduate School of Science, Division of Biological Science, Nagoya University, Professor, 大学院・理学研究科, 教授 (60116663)
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| Co-Investigator(Kenkyū-buntansha) |
OZATO Kenjiro Professor, Bioscience Center, 生物分子応答センター, 教授 (90026790)
KOGA Akihiko Associate Professor, 大学院・理学研究科, 助教授 (80192574)
TAKEDA Hiroyuki Associate Professor, 大学院・理学研究科, 助教授 (80179647)
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| Project Period (FY) |
1997 – 1998
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| Keywords | medaka / Oryzias latipes / transposable element / Tol-2 / tagging |
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| Research Abstract |
Several transposable elements of the terminal-inverted-repeat class have been described in vertebrate genomes. Some of them are considered to be active because of the presence in genic regions or polymorphic situations of inserted elements. However, detection of de novo excision or insertion of the elements has not been successfully done. We have identified a novel terminal-inverted-repeat class transposable element in the medaka fish Oryzias latipes. The element was designated Tol-2. Excision of Tol-2 during embryogenesis was detected by a PCR analysis of a genomic region containing a Tol-2 copy. Tol-2 is 4681 bp in length. Tol-2 contains 4 ORFs which have amino acid sequence similarity with Ac of maize, hobo of Drosophila and Tam3 of snapdragon, which are all active transposable elements. Tol-2 is thus a unique material for establishing a gene tagging system in fish.
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[Publications] Bessho, Y., Tamura, S., Hori, H., Tanaka, H., Ohama, T., Osawa, S.: "Planarian mtDNA and reproductive mode." Mol.Ecol.6. 129-136 (1997)
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[Publications] Hamada K,Tamaki K,Sasado T,Watai Y,Kani S,Wakamatsu Y,Ozato K,Kinoshita M,Kohno R,Takagi S,Kimura M.: "Usefulness of the medaka beta-actin promoter investigated using a mutant GFP reporter gene in transgenic medaka (Oryzias latipes)." Mol.Mar.Biol.Biotechnol.7(3). 173-180 (1998)
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