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1998 Fiscal Year Final Research Report Summary

Real-time monitoring of bio-process by dielectric spectroscopy with inductive probe.

Research Project

Project/Area Number 09555252
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ASAMI Koji  Inst.Chem.Res., Kyoto Univ, Associate Prof., 化学研究所, 助教授 (90127936)

Co-Investigator(Kenkyū-buntansha) WAKAMATSU Hideki  Japan Hewlett-Packard, Senior Researcher, パッカード(株)・電子部品計測事業部技術部, 主任研究員
YONEZAWA Takeshi  Suntory, Senior Researcher, 技術開発センター, 主任研究員
NAKAJIMA Mikio  International Center for Biotech., Osaka Univ., Instruc., 工学部, 助手 (00273590)
SHIOYA Steaki  Dept.of Biotech., Osaka Univ., Prof., 工学部, 教授 (50026259)
Project Period (FY) 1997 – 1998
KeywordsDielectric spectroscopy / Bio-process / Cell growth / Fermentation
Research Abstract

In order to analyze and regulate bio-processes, the real-time monitoring of cell concentration and cell properties is important, for which we have developed a new dielectric measurement technique with an inductive probe. The technique, based on electromagnetic induction, requires no electrode and therefore is free from the problems related to the conventional techniques with electrodes, i.e., electrode polarization and bubble formation on electrodes. In this study, we have examined the feasibility of the technique for the monitoring of cell growth in culture of yeast and filamentous fungi and in whisky and beer fermentation. The results are summarized as follows : (1) The real-time monitoring of yeast growth was successful at concentrations more than 106 cells/mi In synchronized culture, the progression of the cell division cycle was detected as cyclic changes in relative permittivity. (2) The on-line estimation of cell concentration in culture of Manascus (a filamentous fungus) became possible using an appropriate agitation rate and applied frequency. (3) The four characteristic phases in whisky fermentation and the highly synchronized growth in early beer fermentation were excellently traced by the new technique. The real-time dielectric monitoring would be usable for the regulation of the fermentation.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] H.Wakamatsu: "A dielectric spectrometer for liquid using the electromagnetic induction method." Hewlett-Packard Journal. 48. 37-44 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Asami: "Dielectric behavior of budding yeast in cell separation." Biochim.Biophys.Acta. 1381. 234-240 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Asami: "Electrial and morphological changes of human erythrocytes under high hydrostatic pressure followed by dielectric spectroscopy." Annals of Biomedical Engineering. accepted for publication. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Asami: "Real-time monitoring of yeast cell division by dielectric spectroscopy." Biophysical Journal. accepted for publication. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Asami: "Handbook on Ultrasonic and Dielectric Characterization Techniqes for Suspended Particulates (edited by V.A.Hackley and J.Texter)" The American Ceramic Society, 350 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wakamatsu, H.: "A dielectric spectrometer for liquid using the electromagnetic induction method." Hewlett-Packard Journal. 48. 37-44 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asami, K: "Dielectric behavior of budding yeast in cell separation." Biochim.Biophys.Acta. 1381. 234-240 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asami, K.: "Electrical and morphological changes of human erythrocytes under high hydrostatic pressure followed by dielectric spectroscopy." Annals of Biomedical Engineering. accepted for publication. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asami, K.: "Real-time monitoring of yeast cell division by dielectric spectroscopy." Biophysical Journal. accepted for publication. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asami, K.: "Dielectric relaxation spectroscopy of biological cell suspensions." Handbook on Ultrasonic and Dielectric Characterization Techniques for Suspended Particulates.(edited by V.A.Hackley and J.Texter), The American Ceramic Society, Westerville. 333-349 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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