1999 Fiscal Year Final Research Report Summary
Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin
| Project/Area Number |
09555294
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
000079000407000413
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TAKAI Mitsuo Hokkaido Univ., Grad. School of Eng., Pro., 大学院・工学研究科, 教授 (50002019)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBA Toshikazu Hokkaido Univ., Grad. School of Eng., Asso. Pro., 大学院・工学研究科, 助教授 (60241303)
ERATA Tomoki Hokkaido Univ., Grad. School of Eng., Asso. Pro., 大学院・工学研究科, 助教授 (30213581)
MUNEKATA Masanobu Hokkaido Univ., Grad. School of Eng., Pro., 大学院・工学研究科, 教授 (50261326)
TAJIMA Kenji Hokkaido Univ., Grad. School of Eng., Inst., 大学院・工学研究科, 助手 (00271643)
FUJIWARA Masashi Hokkaido Univ., Grad. School of Eng., Inst., 大学院・工学研究科, 助手 (30229075)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Salmon collagen / Squid chitin / Temporarily artificial skin / Human skin cells / Attachment and growth of cells / DNA labeling index / Continuous paper machine / Bio-safety test |
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| Research Abstract |
The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.
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