1999 Fiscal Year Final Research Report Summary
Molecular breeding studies on crop fertility by expressional regulation of respiration-related genes.
| Project/Area Number |
09556002
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Breeding science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIRAI Atsushi Agric. Life Sci., Univ. Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (60023470)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Ikuo Natural Sci., Chiba Univ., Asso. Professor, 大学院・自然科学研究科, 助教授 (50207867)
NAKAZONO Mikio Agric. Life Sci., Univ. Tokyo, Assit. Professor, 大学院・農学生命科学研究科, 助手 (70282697)
TSUTSUMI Nobuhiro Agric. Life Sci., Univ. Tokyo, Asso. Professor, 大学院・農学生命科学研究科, 助教授 (00202185)
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| Project Period (FY) |
1997 – 1999
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| Keywords | respiration / gene expression / mitochondria / 低酸素 |
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| Research Abstract |
Crop fertility depends on energy supply, and energy production is conducted by the respiration. We investigated the effect of oxygen on the expression of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Transcript levels of nuclear-encoded respiratory genes decreased by hypoxic treatment, whereas oxygen deprivation did not affect the expressions of mitochondrial-encoded respiratory genes. The effects of ruthenium red (an inhibitor of CaィイD12+ィエD1 fluxes from organelles) and/or CaClィイD22ィエD2 on plants under hypoxic conditions suggested that CaィイD12+ィエD1 is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase gene (AOX1a), but not for expression of acetaldehyde dehydrogenase genes (ALDH2) and genes involved in the cytochrome respiratory pathway, in rice.
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