1998 Fiscal Year Final Research Report Summary
Construction of effective PCB-degradation bioreactor by modification of regulatory system of PCB-degrading bacterium using genetic engineering.
| Project/Area Number |
09556015
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKAGI Masamichi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (50018339)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMURA Minoru Railway Technical Research Institute. Researcher, 鉄道総合研究所・環境生物工学研究室, 研究員
KIMBARA Kazuhide Railway Technical Research Institute. Head Researcher, 鉄道総合研究所・環境生物工学研究室, 主任研究員
NAGATA Yuji The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (30237531)
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| Project Period (FY) |
1997 – 1998
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| Keywords | PCB / Pseudomonas / GEMs / Regulatory factor / Bioreactor |
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| Research Abstract |
The bph operon of PCB-degraging bacterium, Pseudomonas sp. KKS102 was inducibly expressed by addition of biphenyl. A gene whose deduced amino acid sequence shows similarity to the LysR-type regulator was found just downstream of the bph operon, and named it bphR.The disruption of the bphR gene had no effect on the inducible expression of the bph operon, indicating that the bphR is not a regulator of the bph operon. Four disruptants of bph genes (bphA, bphB, bphC, and bphD) were constructed. The bphA, bphB, and bphC disruptants did not show the inducibility of the bphE gene expression when biphenyl was added. Whlie, the bphD disruptant showed the inducibility. These results indicated that the direct inducer of the bph operon is not biphenyl but meta-cleavage compound which is produced by BphA, BphB, and BphC from biphenyl. Furthermore, cis-element locating just upstream of the bphE gene for a response of biphenyl was identified.
In this study, we revealed a novel regulation mechanism of the bph operon in KKS1O2. We consider that our finding will be able to be applied for the modification of the PCB-degrading bioreactor.
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