1999 Fiscal Year Final Research Report Summary
Technological development of oligosaccharide units as biomaterials of life science
| Project/Area Number |
09556021
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Shizuoka University |
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Principal Investigator |
USUI Taichi Shizuoka University, Faculty of Agriculture, Professor, 農学部, 教授 (50111802)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yasuo University of Shizuoka, Faculty of Pharmaceuitical, Professor, 薬学部, 教授 (00046278)
KOBAYASHI Kazukiyo Nagoya University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (10023483)
MURATA Takeomi Shizuoka University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (30273171)
AJISAKA Katsumi Meiji Milk Products Co., Ltd., Meiji Institute of Health Science, Research Manager, ヘルスサイエンス研究所, 室長
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| Project Period (FY) |
1997 – 1999
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| Keywords | Glycotechnology / Biomaterial / Glycoconjugate / Oligosacchride unit / Enzymatic synthesis / Glycopolymer / Molecular recognition / Molecular design |
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| Research Abstract |
Our purpose is to prepare oligosaccharide units library folowing practical synthesis of biologically important oligosaccharides essentially for developing glycobology and glycottechnolog. The synthesis of possible array of linkages of (1-3), (1-4) and (1-6) of β-D-Gal-D-GlcNAc and β-D-Gal-D-GalNAc was done by using some readily available β-D-galactosidases. The preparation of the galactosyl-disaccharide units library led to the enzymatic synthesis of trisaccharides containing GlcNAc; β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-GlcNAc as a common unit of polylacto- saminoglycan and β-D-Gal-(1-3)-[β-D-GlcNAc-(1-6)]-α-D-GalNAc-OCィイD26ィエD2HィイD24ィエD2NOィイD22ィエD2-p as a carbohydrate unit of mucin type 2 core. Transglycosylation of L-Fuc to LacNAc and lactose utilizing α-L-fucosidases also produced α-L-fucosyl-N-acetyllactosamine and 3'-O-α-L-fucosyllactose. Furthermore, as a synthetic strategy of human milk oligosaccharide, lacto-N-tetraose (β-D-Gal-(1-3)-β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-D-Glc)and lacto-N-neotetraose(β-D-Gal-(1-4)-β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-D-Glc) were practically synthesized by a combination of glycosyltransferase catalyzed reaction and glycosidase-catalyzed transglycosylation from lactose as an initial substance.
Such synthetic oligosaccharides available in large amounts were used to synthesize glycopolymer. chemo-enzymatic synthesis of glycopeptides carrying LacNAc unit as a model of asialoglycoprotein, and β-D-Gal-(1-3)-α-D-GalNAc and α-NeuAc-(1-3)-β-D-Gal-(1-3)-α-D-GalNAc units as models of mucin-type glycoproteins was carried out by introducing these oigosaccharides as side chain into polyglutamic acid as main chain. The artgificial glycopolypeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of glycoprotein-lectin interactions.
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