1999 Fiscal Year Final Research Report Summary
Development of reagents for effective genetic modification of plants
| Project/Area Number |
09556024
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | OKAYAMA UNIVERSITY |
|---|
Principal Investigator |
KANZAKI Hiroshi Fac. Agriculture, Okayama Univ., Associate Prof., 農学部, 助教授 (60183787)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OBATA Tokio Ube Research Center, Ube, Industries, Chief Researcher, 宇部研究所, 主任研究員
NITODA Teruhiro Center of Instrumental Analysis, Assistant Prof., 機器分析センター, 助手 (80284090)
|
|---|
| Project Period (FY) |
1997 – 1999
|
| Keywords | crown gall / Agrobacterium / plant transformation inhibitor / inhibition of bacterial attachment / acidic polysaccharide / cellulose biosynthesis / cyclic b1, 2-glucan / adhesin |
|---|
| Research Abstract |
Tow novel geometrical isomers of oxazolomycin, oxazolomycins B (2) and C(3), were isolated from the fermentation broth of an oxazolomycin- producing strain, Streptomyces albus JA3453. The configurations of the triene moieties of 2 and 3 are (4'E, 6'E, 8'E) and (4'Z, 6'E, 8'E), respectively, while that of oxazolomycin (1) is (4'Z, 6'Z, 8'E). 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 μg/disk) as oxazolomycin. 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity. The newly established GUS expression bioassay of the plant callus extracts revealed that the extracts of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. We found that the inhibitory activity of oxazolomycin oxazolomycin diacetate against plant transformation was due to its inhibitory effect against the attachment of A. tumefaciens to host plants. Furthermore, oxazolomycin diacetate was found to exhibit an inhibitory activity against the rhicadhesin-mediated agrobacterial attachment to plants, not the biosynthesis of cellulose and β-1, 2-glucan which have been reported to be involved in the agrobacterial attachment. And we found that a novel bacterial acidic polysaccharide from a soil bacterium exhibited plant transformation enhanced plant transformation due to its stimulatory effect for the attachment of A. tumefaciens to host plants.
|
