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1998 Fiscal Year Final Research Report Summary

Development of a detection method for food-derived toxicants by using a monolayer-cultured human intestinal epithelial cells

Research Project

Project/Area Number 09556026
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 食品科学・栄養科学
Research InstitutionThe University of Tokyo

Principal Investigator

SHIMIZU Makoto  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (30114507)

Co-Investigator(Kenkyū-buntansha) WATANABE Hirohito  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Rese, 大学院・農学生命科学研究科, 助手 (20270895)
Project Period (FY) 1997 – 1998
Keywordsintestinal epithelial cell / detection method for toxicants / transepithelial electrical resistance / monolayer culture / tight junction
Research Abstract

Development of a novel experimental method for the detection of toxicants in food was attempted by using a monolayer culture system with intestinal cpithelial cells. A variety of toxicants seemed to be detectable by measuring transepithelial electrical resistance (TER) of the human intestinal Caco-2 cell monolayer after exposure to the toxicants. By adding such toxicants as benzalkonium chloride(a membrane perturbing toxicant with surface activity), flammutoxin and discodermin A (membrane pore-forming toxicants), mycalolide B and cytochalasin D (actin filament-disturbing toxicants), caliculin A and okadaic acid(protein phosphatase inhibitory toxicants)to the apical chamber of the Caco-2 cell monolayer, rapid decrease in TER was observed. Sodium azide, a metabolic inhibitor, also decreased the TER in a time-dependent manner. Although the mechanisms for the cell damage caused by the above-mentioned toxicants are different each other, the disruption or disturbance of the cytoskeletal stru … More cture may occur as a common change. The changes in the cytoskeletal structure may induce the reorganization of tight junctional molecules, resulting in the increase of the tight junctional permeability. The TER decrease commonly observed as a response to cytotoxic substances is thought to reflect the changes in cytockeletal/tight junctional structure. On the other hand, such bacteria toxins as verotoxin 1&2 and a marine toxin, onnamide A(protein biosynthesis inhibitory toxicants)did not affect the TER of the Caco-2 monolayer. The present detection method seems to be not applicable for the toxins which take longer time to express their toxicity. The decrease in TER of the cell monolayer was accompanied with the increase in the permability for proteins. This system is therefore thought to be useful not only for the detection of substances with cytotoxicity but also for the detection of harmful substances which disrupt the intestinal barrier and enhance the absorption of food allergens and xenobiotics. Less

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] A.Narai: "Rapid decrease in transepithelial electrical resistance of human intestinal Caco-2 cell monolayers by cytotoxic membrane perturbeuts" Toxicology in Vitro. 11. 347-354 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Narai, S.Arai and M.Shimizu: "Rapid decrease in transepithelial electrical resistance of human intestinal Caco-2 cell monolayers by cytotoxic membrane perturbents" Toxicology in Vitro. 11. 347-354 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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