| Co-Investigator(Kenkyū-buntansha) |
KAMITAKAHARA Hiroshi Kyoto Univ., Dept.of Agriculture, Instructor, 農学研究科, 助手 (10293911)
KAWADA Toshinari Kyoto Univ., Tottori University, Dept.of Agriculture, Associate Prof., 農学部, 助教授 (40214655)
KAWAMOTO Haruo Kyoto Univ., Dept.of Energy Science, Associate Prof., エネルギー研究科, 助教授 (80224864)
UMEZAWA Toshiaki Kyoto Univ., Wood Research Institute, Associate Professor, 木質科学研究所, 助教授 (80151926)
MINATO Kazuya Kyoto Univ., Dept.of Agriculture, Associate Professor, 農学研究科, 助教授 (10026601)
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| Research Abstract |
Natural and chemically synthesized polysaccharides were sulfated to investigate its biological activities. First, heparanase inhibition activities were investigated, It was found that (l(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (l(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate have heparanase inhibition activities. Then, anticoagulant activities were measured by an APTT (activated partial thromboplastin time). Among the tested compounds, (1(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate have low anticoagulant activities. Furthermore, inhibition activities of B 16BL6 lung metastasis by the four polysaccharides, i. e., (1(]SY.tri-substituted left.[)5)- beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, xylan sulfate, and curdlan sulfate is in the order : (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate> (l(]SY.tri-substituted left.[)5)-beta-D-Glucofuranan sulfate> xylan sulfate, curdlan sulfate. It was found that (l(]SY.tri-substituted left.[)5)-beta-D-glucofuranan sulfate, (1(]SY.tri-substituted left.[)5)-beta-D-galactofuranan sulfate, and xylan sulfate have not only low anticoagulant activities but also high inhibition actibities of Bi 6BL6 lung metastasis.
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