1999 Fiscal Year Final Research Report Summary
PRODUCTION OF RECOMBINANT GONADOTROPINS (FSH AND LH) AND APPLICATION FOR CONTROL IN REPRODUCTION
| Project/Area Number |
09556065
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | Gunma University |
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Principal Investigator |
KATO Yukio GUNMA UNIVERSITY, INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION, ASSOCIATE PROFESSOR, 生体調節研究所, 助教授 (30114177)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Satoshi GUNMA UNIVERSITY, INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION, POSTDOCTRAL FELLOW, 生体調節研究所, 特別研究員
IHARA Takeshi NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE, RESEARCHER, 研究員 (70150109)
MORI Junichi KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCIENCES, HONORARY PROFESSOR, 獣医畜産学部, 客員教授 (90167685)
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| Project Period (FY) |
1997 – 1999
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| Keywords | PITUITARY / RECOMBINANT DNA / GONADOTROPIN / CLONING / GENE / BACUROVIRUS / REPRODUCTION / FERTILIZATION |
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| Research Abstract |
(1) RECOMBINANT PORCINE BEEN SUCCESSIVELY PRODUCED AND THE BIOCHEMICAL CHARACTERISTICS WERE EXAMINED. THE BIOLOGICAL ACTIVITIES WERE OBSERVED.
(2) EXPRESSION VECTOR FOR PORCINE LH WAS CONSTRUCTED AND EXAMINED LH PRODUCTION. THE RESULT SHOWED THE LOW LEVEL OF EXPRESSION AS FSH.
(3) ANTIBODY FOR RECOMBINANT PORCINE FSH WAS PRODUCED. THE ANTISERUM REACTED WITH ONLY PORCINE FSH IN COMPETITIVE IMMUNOASSAY SYSTEM, INDICATING THAT THE ANTISERUM IS SPECIES-SPECIFIC. IN THE NON-COMPETITIVE USAGE SUCH AS IMMUNOHISTOCHEMISTRY, THE ANTI-RECOMBINANT PORCINE FSH ANTISERUM GAVE POSITIVELLY IMMUNOREACTIVE PITUITARY CELLS FOR SEVERAL MAMMALS.
(4) USAGE OF RECOMBINANT PORCINE FSH FOR IN VITRO FERTILIZATION WAS EXAMINED. RECOMBINANT PORCINE FSH INDUCED THE GVBD OF PORCINE OOCYTES BUT NOT THAT OF BOVINE OOCYTES.
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Research Products
(16 results)
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[Publications] Inaba, T., Mori, J., Ohmura, M., Tomizawa, K., Kato, Y., Ihara, T., Sato, I. and Ueda, S.: "Baculovirus-insect cell production of bioactive porcine FSH."Theriogenology. 47. 491-499 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kato, Y., Sato, I., Ihara, T., Tomizawa, K., Mori, J., Geshi, M., Nagai, T., Okuda, K., Kato, T. and Ueda, S.: "Expression of biologically active porcine follicle stimulating hormone in insect cells bearing a baculovirus vector and its purification."J. Mol. Endocrinol.. 20. 55-65 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Inaba, T., Mori, J., Ohmura, M., Tani, H., Kato, Y., Tomizawa, K., Kato, T., Ihara, T., Sato, I. and Ueda, S.: "Recombinant porcine follicle stimulating hormone produced in baculovirus-insect cells induces rat ovulation in vivo and gene expression of tissue plasminogen activator in vitro."Res. Vet. Sci.. 64. 25-29 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Kato, Y., Koike, Y., Ogawa, S., Tomizawa, K., Hosaka, K., Tanaka, S. and Kato, T.: "Presence of activating transcription factor 4 (ATF4) in porcine anterior pituitary."Mol. Cell. Endocrinol.. 154. 151-159 (1999)
Description
「研究成果報告書概要(欧文)」より
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