1998 Fiscal Year Final Research Report Summary
Regulation of Disease Susceptibility through Gene Introduction of Transcriptiom Factors.
| Project/Area Number |
09557010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAMAMOTO Masayuki University of Tsukuba, Institute of Basic Medical Sciences, Professor., 基礎医学系, 教授 (50166823)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Satoru University of Tsukuba, Institute of Basic Medical Sciences, Lecturer., 基礎医学系, 講師 (50271896)
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| Project Period (FY) |
1997 – 1998
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| Keywords | c-Maf / transcription factor / transgenic mouse / T lymphocyte / autoimmune disease / differentiation |
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| Research Abstract |
The susceptibility to various diseases is closely related to the balance between T lymphocyte subpopulations (Th1 cells vs. Th2 cells). According to the recent report, Th2 cells were induced by c- Maf which activates the transcription of IL-4 gene. On the basis of these results, we intended to regulate disease susceptibility by utilizing c-Maf for changing the balance between Th1 and Th2 cells.
(1) Generation of transgenic mice which express c-Maf in T lymphocyte lineage. Transgenic mice were generated which express c-Maf under the gene regulatory system of CD2 or LCK in T lymphocytes. Th2 cells were dominant in the mice from the serological analysis. The mice also frequently suffered from lymphoma after 8-10 months old. We are planning to introduce the transgene to the background which shows spontaneous onset of autoimmune diseases and to see whether the transgene will change the disease susceptibility.
(2) Gene disruption of mouse c-maf gene. We completed gene disruption of mouse c-maf gene. c-Maf null mice were mostly lethal at the late stage of embryogenesis and showed defective lens formation. We will search for the influence on T lymphocyte differentiation in the mutant mice.
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