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1998 Fiscal Year Final Research Report Summary

A STUDY OF GENES DIFFERENTIALLY EXPRESSED BY OSTEOCLASTOGENIC MOUSE STROMAL CELLS

Research Project

Project/Area Number 09557021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Experimental pathology
Research InstitutionKOBE UNIVERSITY

Principal Investigator

KITAZAWA Riko  KOBE UNIVERSITY SCHOOL OF MEDICINE,2ND DEPARTMENT OF PATHOLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (00273780)

Co-Investigator(Kenkyū-buntansha) KITAZAWA Sohei  KOBE UNIVERSITY SCHOOL OF MEDICINE,2ND DEPARTMENT OF PATHOLOGY,ASSISTANT PROFESS, 医学部, 講師 (90186239)
Project Period (FY) 1997 – 1998
Keywordspostmenopausal osteoporosis / osteoclast / stromal cell / RANKL / TRANCE / ODF / OPGL / promoter / in situ hybridizatin
Research Abstract

Osteoclasts are multinucleated cells derived from hemopoietic cells. Osteoblastic/ stromal cell lineage contributes to the terminal differentiation of osteoclast precursor s through the direct cell-to-cell interaction mechanism (Takahashi et al., 1988). ST2 cells, mouse stromal cell line, support osteoclastogenesis in the coculture with hematopoietic cells in the presence of Vitamin D3 and Dexamethasone. We found the phenomenon of the passage-dependent reduction of osteoclastogenesis in the co-culture system of ST2 and hemopoietic cells. In this study we attempted to isolate the molecules of which gene expression is associated to the passage-dependent capability of supporting osteoclastogenesis, and got 1 .5 kb cDNA of that molecule.
Recently osteoclast differentiation factor (RANKL/ TRANCE/ OPGL/ ODF) was identified by others (H.Yasuda et al., P.N.A.S., 1998 ; D.Lacy et al., Cell, 1998). We then cloned and analyzed of 5-flanking basic promoter region of the mouse RANKL gene and attempted to elucidate the regulatory mechanism of RANKL gene expression in relation to the passage-dependent capability of ST2 cells to support osteoclastogenesis. To assess the expression of genes involved in osteoclastogenesis on the bone tissues, we set up the system of in situ hybridization using decalcified bone specimen. We have presented our data at various international as well as domestic meetings, and publish a lot of scientific papers for academic journals.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kitazawa R et al.: "Promoter structure of mouse RANKL/TRANCE/OPGL/ODF gene." Biochemica et Biophysica Acta. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitazawa R et al.: "Expression of bone morphogenetic proteins(BMPs)in fractured mouse bone tissue:In situ hybridization with polymerase chain reaction derived antisense DNA probe." Acta Histochemica et Cytochemica. 31・3. 231-236 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitazawa S et al.: "Promoter stucture of human sonic hedgehog gene." Biochemica et Biophysica Acta. 1443. 358-363 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitazawa S et al.: "In situ hybridization with polymerase chain reaction-derived single-stranded DNA probe and S1 unclease." Histochem Cell Biol. 111・1. 7-12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitazawa R et al.: "In situ detection of parathyroid hormone-related protein(PTHrP)in ovarian clear cell carcinoma." Human Pathol. 28・3. 379-382 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishida K et al.: "Identification of regulatory elements of human a6 integrin subunit gene." BBRC. 241・2. 258-263 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 北澤荘平他: "In situ hybridization技法" 学際企画, 220 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishida K,Kitazawa R,Mizuno K,Maeda S,Kitazawa S.: "Identification of regulatory elements of human a6 integrin subunit gene." BBRC. 241 (2). 258-263 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitazawa R,Kitazawa S,Matsui T,Maeda S: "In situ detection of parathyroid hormone-related protein (PTHrP) in ovarian clear cell carcinoma." Human Pathol.28 (3). 379-382 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitazawa R,Kitazawa S,Kashimoto H,Maeda S: "Expression of bone morphogenetic proteins (BMPs) in fractured mouse bone tissue : In situ hybridization with polymerase chain reaction (PCR)-derived antisense DNA probe." Acta Histochemica et Cytochemica. 31, (3). 231-236 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitazawa S,Kitazawa R,Tamada H,Maeda S: "Promoter structure of human sonic hedgehog gene." Biochemica et Biophysica Acta. 1443. 358-363 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamada H,Kitazawa R,Gohji K,Kamidono S,Maeda S,Kitazawa S: "Molecular cloning and analysis of the 5'-flanking region of the human bone morphogenetic protein-6 (BMP-6)." Biochemica et Biophysica Acta. 1395. 247-251 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami R,Kitazawa R,Maeda S,Kitazawa S: "Analysis of 5'-flanking region of human smad4 (DPC4) gene." Biochemica et Biophysica Acta. 1443. 182-185 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsutsuji M,Kitazawa R,Tamada H,Maeda S,Kitazawa S: "Molecular cloning and characterization of human mxil gene promoter." J Biochem Mol Biol Biophys. 2. 59-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashimoto H,Kitazawa R,Maeda S,Mizuno K,Kitazawa S: "Modulation of osteonectin and osteopontin expression during fracture healing of mouse bone tissue." Acta Histochem Cytochem. 3. 501-508 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanatani M,Sugimoto T,Takahashi Y,Kaji H,Kitazawa R,Chihara K: "Estrogen via the estrogen receptor blocks cAMP-mediated parathyroid hormone (PTH)-stimulated osteoclast formation." J Bone Miner Res. 13. 854-861 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muraoka A,Suehiro I,Fujii M,Ueno H,Hayashi S,Shimizu K,Kitazawa R,Kitazawa S,Murakami K.: "Type IIa early gastric cancer with proliferation of xanthoma cells." J Gastroenterol. 33. 326-329 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kakizaki S,Ohya N,Yoshinaga T,Higuchi T,Kitazawa R,Takayama H,Takagi H,Nagamine T,Mori M.: "Undifferentiated pancreatic cancer associated with humoral hypercalcemia of malignancy." Jpn J Clin Oncol. 28. 563-566 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitazawa S,Kitazawa R,Maeda S: "In situ hybridization with polymerase chain reaction-derived single-stranded DNA probe and S1 unclease." Histochem Cell Biol. 111. 7-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitazawa R,Kitazawa S,Maeda S: "Promoter structure of mouse RANKL/TRANCE/OPGL/ODF gene." Biochemica et Biophysica Acta. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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