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1998 Fiscal Year Final Research Report Summary

STUDY ON THE ANALYSIS OF TAXONOMIC DISTANCE AMONG

Research Project

Project/Area Number 09557024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Bacteriology (including Mycology)
Research InstitutionGIFU UNIVERSITY

Principal Investigator

EZAKI Takayuki  GIFU UNIV.MICROBIOLOGY,PROFESSOR, 医学部, 教授 (90151977)

Co-Investigator(Kenkyū-buntansha) KAWAMURA Yoshiaki  GIFU UNIV.MICROBIOLOGY,ASSISTANT, 医学部, 助手 (80262757)
Project Period (FY) 1997 – 1998
Keywordstaxonomy / species definition / DNA hybrid / ribosome / Phylogeny / Liquid phase hybridization
Research Abstract

Prokaryotes were phylogenetically classified by 16SrRNA sequences and genetic distance among independent species were quantitatively measured simply by comparing their 16SrRNA differences. However, it become clear that some established species shares same 16SrRNA sequences.
In this case, we need to use other method to differentiate closely related species. Quantitative DNA/DNA hybridization method is a officially accepted method to define a bacterial species. However, this method is difficult to perform and DNA used for this method is often cotaminated with polysaccharide, which interfers quantitative analysis of DNA/DNA hybrid. We established several parameters to evaluate DNA purity and a rapid measuring method of guanine plus cytosine contents of DNA to define DNA/DNA hybridization experiments. RNA and polysaccharide contamination was successfully predicted by measuring absorbance of 235/260 nm and Anthrone quantitation method of sugars. Guanine plus cytosine content of DNA was instantly measured by Light cycler with SYBR Green 1 fluorescence. This information is very useful to define quantitative DNA/DNA hybridization at optimal temperature. Trials to differentiate strains within a species were successfully introduced by many scientists. We used Ceul restriction enzyme to evaluate distances among strains. This method has a advantage because bands generated by the Ceul digestion indicate numbers of copies of ribosomal RNA operon. From fragment analysis of generated size offered useful information to analyze gene recombination history.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kawamura,Y,et al.: "Genetic Approaches to the Identification of the Mitis Group within the genus Streptococcus." J.Microbiol.155in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yabuuchi,E.et al.: "Emendation of Genus Achromobacter and Achromobacter xylosoxidans(Yabuuchi and Yano) and proposal of Achromobacter ruhlandii(Packer and Vishniac) comb.nov.〜" Microbiol.Immunol.42・2. 429-438 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hou XG,et al.: "Description of Arthrobacter creatinolyticus sp.nov.,isolated from human urine." Int.J.Syst.Bacteriol. 48. 423-429 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamura,Y,et al.: "Streptococcus peroris sp.nov.and Streptococcus infantis sp.nov.; new members of the Streptococcus mitis group,isolated〜" Int.J.Syst.Bacteriol. 48. 921-927 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sultana,F.,et al.: "Determination of 23S rRNA Sequences from members of genus Streptococcus and Characterization of genetically distinct organisms previously identified as members of〜" FEMS Microbiol.Lett.158. 223-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hou,X.G.,et al.: "Genetic Identification of Members of the genus Corynebacterium at genus and species levels.with 16S rDNA targeted probes." Microbiol.Immun.41・6. 453-460 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamura, Ezaki: "Genetic approaches to the identification of the mitis group within the genus Streptococcus." J.Microbiol.155 : (in Press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura, Y., X.G.Hou, , Y.Todome, F.Sultana, K.Hirose, K.S.E.Shu T.Ezaki and H.Okuni: "1998.Streptococcus peroris sp.nov.and Strepto-coccus infantis sp.nov. ; new members of the streptococcus mitis group, isolated from human clinical specimens." Int.J.Syst.Bacteriol. 48. 921-927 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yabuuchi, E., Y.Kawamura, Y.Kosako, and T.Ezaki.: "Emendation of Genus Achromobacter and Achromobacter xylo-soxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb.nov., Achromobacter piechaudii (Kiredjian et al.) Comb.nov., and Achromobacter xylosoxidans subsp.denitrificans (Ruger and Tan) comb.nov." Microbiol.Immunol.42. 429-438 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hou XG,Kawamura Y,Sultana F,Shu S,Hirose K,Goto K,Ezaki T @: "Description of Arthrobacter creatinolyticus sp.nov., isolated from human urine." Int.J.Syst.Bacteriol. 48. 423-429 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sultana, F._AY.Kawamura, T.Ezaki: "Determination of 23S rRNA Sequences from members of genus Streptococcus and Characterization of genetically distinct organisms previously identified as members of Streptococcus anginosus group. FEMS Microbiol.Lett.158 : 223-230." FEMS Microbiol.Lett. 158. 223-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura Y., X.G.Hou, F.Sultana, K.Hirose, M.Miyake, S.Shu, and T.Ezaki: "Distribution of Staphylococcus species among human clinical specimens and emended description of Staphylococcus caprae.J.Clin.Microbiol.36 : 2038-2042." J.Clin.Microbiol.36. 2038-2042 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hou, X.G., Y.Kawamura, and T.Ezaki, et al.: "Genetic Identifi-cation of Members of the genus Corynebacterium at genus and species levels.with 16S rDNA targeted probes." Microbiol.Immun.41 (6). 453-460 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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