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1998 Fiscal Year Final Research Report Summary

Monitoring method of human cytomegalovirus latent infection and viral reactivation

Research Project

Project/Area Number 09557028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

KONDO Kazuhiro  Osaka University Medical School, Associate Professor, 医学部, 助教授 (70234929)

Co-Investigator(Kenkyū-buntansha) AONO Toshiya  Medical Research Laboratory Toyobo Research Center, Researcher, 総合研究所・メディカル研究所, 研究員
TAYA Keiko  Osaka University Medical School, Associate Professor, 医学部, 助手 (80263276)
INAGI Reiko  Osaka University Medical School, Associate Professor, 医学部, 助手 (50232509)
YAMANISHI Koichi  Osaka University Medical School, Professor, 医学部, 教授 (10029811)
Project Period (FY) 1997 – 1998
Keywordscytomegalovirus / latency / reactivation / NASBA
Research Abstract

Human cytomegalovirus (HCMV) is a significant cause of morbidity and mortality in immunocompromised patients, such as organ or bone marrow transplant (BMT), AIDS patients and developing neonates. Some antiviral drugs, such as ganciclovir and foscarnet, are useful for treating HCMV diseases, but these drugs must be used carefully because of their side effects. Therfore, it is important to establish a sensitive system by which to monitor HCMV infection.
In the diagnosis of HCMV infection, it is very important to distinguish symptomatic from asymptomatic infection. The nucleic acid sequence-based amplification (NASBA) technique was compared with single and nested polymerase chain reaction (PCR) methods.
For NASBA detection, the beta 2.7 transcript was chosen as a target because of its abundant active HCMV-specific expression. Of 20 pediatric BMT recipients, 8 developed HCMV-related clinical symptoms. The clinical sensitivities and specificities were 50% and 100% for single PCR, 100% and 67% for nested PCR, and 100% and 83% for NASBA, respectively. Follow-up of HCMV infections in pediatric BMT recipients showed that NASBA could both detect viral transcript prior to the onset of clinical symptoms and reflect clinical improvement due to antiviral therapy. These data suggest that NASBA should be useful for both predicting HCMV disease development and monitoring the effect of antiviral therapy.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Aono T、et al.: "Monitoring of human cytomegalovirus infections in pediatric bone marrow transplant recipients by nucleic acid sequence-based amplification."J Infect Dis.. 178. 1244-1249 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori Y、et al.: "Analysis of human herpesvirus 6 U3 gene,which is a positional homolog of human cytomegalovirus UL 24 gene."Virology.. 249. 129-139 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Na Ayuthaya PI、et al.: "The association of skin deseases with human herpesvirus 8 infection in HIV carriers."Arch Virol.. 143. 1881-1892 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujisaki H、et al.: "Detection of human herpesvirus 7(HHV-7)DNA in breast milk by polymerase chain reaction and prevalence of HHV-7 antibody in breast-fed and bottle-fed"J Med Virol.. 56. 275-279 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isegawa Y、et al.: "Human herpesvirus 6 open reading frame U12 encodes a functional beta-chemokine receptor."J Virol.. 72. 6104-6112 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aono T, et al.: "Monitoring of human cytomegalovirus infections in pediatric bone marrow transplant recipients by nucleic acid sequence-based amplification"J Infect Dis. Nov;178(5). 1244-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mon Y, et al.: "Analysis of human herpesvirus 6 U3 gene, which is a positional homolog of human cytomegalovirus UL 24 gene"Virology. Sep 15;249(1). 129-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Na Ayuthaya PI, et al.: "The association of skin diseases with human herpesvirus 8 infection in HIV carriers"Arch Virol.. 143(10). 1881-92 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujisaki H, et al.: "Detection of human herpesvirus 7 (HHV-7) DNA in breast milk by polymerase chain reaction and prevalence of HHV-7 antibody in breast-fed and bottle-fed children"J Med Virol.. Nov;56(3). 275-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isegawa Y, et al.: "Human herpesvirus 6 open reading frame U12 encodes a functional beta-chemokine receptor"J Virol.. Jul;72(7). 6104-12 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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