1998 Fiscal Year Annual Research Report

B細胞性腫瘍に対する遺伝子免疫療法の開発

Research Project

Project/Area Number	09557083
Research Institution	NAGOYA UNIVERSITY
Principal Investigator	齋藤英彦名古屋大学, 医学部, 教授 (20153819)
Co-Investigator(Kenkyū-buntansha)	安部明弘名古屋大学, 医学部, 医員都築忍名古屋大学, 医学部, 医員恵美宣彦名古屋大学, 医学部, 助手 (30185144)
Keywords	Idiotype / 遺伝子ワクチン / B細胞性腫瘍
Research Abstract	1. 安全なマテリアルを作成するための準備 Naked DNA法のマテリアルとしてplasmid DNAを作成し、エンドトキシン等を定量し安全性を確認した。 DNAのdegradationを防ぐために糖鎖にてDNAを修飾することにより、DNAase耐性ののマテリアルを作ることに成功した。昨年秋に完成した名古屋大学医学部・遺伝子調製室を用いてマテリアル管理をGLPレベルにて行うことを準備中である。 2. 非ウイルスベクターによる効率の良い遺伝子導入法の開発 SFVの構造を基本構造とした新しいDNA型発現 vector、pSFV3-CMV-pAを作製した。このvectorはSFV由来のreplicaseの機能を介し、哺乳動物細胞内で外来遺伝子産物を大量に発現した。従来のSFV由来の発現vectorと異なり、in vitro transcriptionによるrecombinant RNAの合成が不要で、p1asmid DNAのままで遺伝子導入に用いることが可能である。導入後は一過性に大量のタンパクを産生し、その後導入細胞は死滅する。この特徴を生かした遺伝子免疫法などへの応用が検討している。 3. 実際の臨床に向けて、患者登録、プロトコールの作成既に患者検体を100例近く集め、遺伝子治療の適応になる患者の遺伝子配列、血清、細胞保存を開始している。臨床プロトコールの作成も進んでおり現在のVersionを研究成果報告書に添付している。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] KOHNO Akio: "Semliki Forest virus-based DNA expression vector:transient protein production followed by cell death" Gene Therapy. 5. 415-418 (1998)
[Publications] IMAIZUMI Kazuyoshi: "Bystander Tumoricidal Effect and Gap Junctional Communication in Lung Cancer Cell Lines" Am.J.Respir.Ccll Mol.Biol.18. 205-212 (1998)
[Publications] ITOU Toshihide: "Recombinant abeno-associated virus-mediated gene transfer into human leukemia cell lines" International Journal of HEMATOLOGY. 67. 27-35 (1998)
[Publications] ABE Kihiro: "Polybrene increases the efficiency of gene transfer by lipofection" Gene Therapy. 5. 708-711 (1998)
[Publications] Akira Katsumi: "The Carboxy1-Terminal Region of Protein C Is Essential for Its Secretion" Blood. 91(10). 3784-3791 (1998)
[Publications] KOJI YAMAMOTO: "Renal Expression of Fibrinolytic Genes and Tissue Factor in a Murine Model of Renal Disease as a Function of Age" SEMINARS IN THROMBOSIS AND HEMOSTASIS. 24(3). 261-268 (1998)
[Publications] AKIHIRO ABE: "Enhanced Gene Transfer with Fusogenic Liposomes Containing Vesicular Stomatitis Virus G Glycoprotein" JOURNAL OF VIROLOGY. 72(7). 6159-6163 (1998)
[Publications] AKIHIRO ABE: "In Vitro Cell-Free Conversion of Noninfectious Moloncy Retrovirus Particles to an Infectious Form by the Addition of the Versicular Stomatitis Virus Surrogate Envelope G Protein" JOURNAL OF VIROLOGY. 72(8). 6356-6361 (1998)
[Publications] Masanobu Kasai: "Immune response of post-transplant peripheral Tlymphocytes against the patient pre-B cell line NAGL-1" International Journal of Hematology. (in press).
[Publications] Hirokazu Mizuno: "Successful Culture and Sustainability In Vivo of Gene-modified Human Oral Mucosal Epithelium" Human Gene Therapy. (in press).
[Publications] Nobuhiko Emi: "CD4 and CD56 positive T cell line,MTA,established from NK-like T-cell leukemia/Lymphoma" International Journal of Hematology. (in press).

1998 Fiscal Year Annual Research Report

B細胞性腫瘍に対する遺伝子免疫療法の開発

Principal Investigator

齋藤 英彦 名古屋大学, 医学部, 教授 (20153819)

Research Products

[Publications] KOHNO Akio: "Semliki Forest virus-based DNA expression vector:transient protein production followed by cell death" Gene Therapy. 5. 415-418 (1998)

[Publications] IMAIZUMI Kazuyoshi: "Bystander Tumoricidal Effect and Gap Junctional Communication in Lung Cancer Cell Lines" Am.J.Respir.Ccll Mol.Biol.18. 205-212 (1998)

[Publications] ITOU Toshihide: "Recombinant abeno-associated virus-mediated gene transfer into human leukemia cell lines" International Journal of HEMATOLOGY. 67. 27-35 (1998)

[Publications] ABE Kihiro: "Polybrene increases the efficiency of gene transfer by lipofection" Gene Therapy. 5. 708-711 (1998)

[Publications] Akira Katsumi: "The Carboxy1-Terminal Region of Protein C Is Essential for Its Secretion" Blood. 91(10). 3784-3791 (1998)

[Publications] KOJI YAMAMOTO: "Renal Expression of Fibrinolytic Genes and Tissue Factor in a Murine Model of Renal Disease as a Function of Age" SEMINARS IN THROMBOSIS AND HEMOSTASIS. 24(3). 261-268 (1998)

[Publications] AKIHIRO ABE: "Enhanced Gene Transfer with Fusogenic Liposomes Containing Vesicular Stomatitis Virus G Glycoprotein" JOURNAL OF VIROLOGY. 72(7). 6159-6163 (1998)

[Publications] AKIHIRO ABE: "In Vitro Cell-Free Conversion of Noninfectious Moloncy Retrovirus Particles to an Infectious Form by the Addition of the Versicular Stomatitis Virus Surrogate Envelope G Protein" JOURNAL OF VIROLOGY. 72(8). 6356-6361 (1998)

[Publications] Masanobu Kasai: "Immune response of post-transplant peripheral Tlymphocytes against the patient pre-B cell line NAGL-1" International Journal of Hematology. (in press).

[Publications] Hirokazu Mizuno: "Successful Culture and Sustainability In Vivo of Gene-modified Human Oral Mucosal Epithelium" Human Gene Therapy. (in press).

[Publications] Nobuhiko Emi: "CD4 and CD56 positive T cell line,MTA,established from NK-like T-cell leukemia/Lymphoma" International Journal of Hematology. (in press).

齋藤英彦名古屋大学, 医学部, 教授 (20153819)