1999 Fiscal Year Final Research Report Summary
Biomaterial having active sites of collagen-binding adhesion molecules (CMP and RGD-CAP)
| Project/Area Number |
09557146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KATO Yukio Hiroshima University, Faculty of Dentistry, Professor, 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Yasunori Keio University, School of Medicine, Professor, 医学部, 教授 (00115221)
NOSIRO Matsuhide Hiroshima University, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (00144858)
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| Project Period (FY) |
1997 – 1999
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| Keywords | CMP / RGD-CAP / chondrocyte / integrin / collagen / biomatenal / 生体材料 |
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| Research Abstract |
The CMP coating enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The effect of CMP on the cell spreading was synergistically increased by type 11 collagen. We also showed that 1251-CMP selectively binds to alphalbetal integrin directly using monoclonal antibodies to various integrin subunits. In addition, we cloned cDNAS for rabbit matrilin1 (CMP) and rabbit matrilin3 to identify active sites for the stimulation of cell adhesion.
The RGD-CAP coating also enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The RGD sequence near the C-terminal was not essential for the adhesion activity. Studies with monoclonal antibodies to various integrin subunits showed that RGD-CAP is a novel ligand for alpha1beta1 integrin that does not bind to the RGD motif. We have shown that at least two repeated structures within the RGD-CAP molecule are required for the stimulation of cell adhesion. These findings will be useful for clinical application of CMP and RGD-CAP, as well as their fragments, as biomaterials in tissue engineering.
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