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1998 Fiscal Year Final Research Report Summary

Developmentofan New Implant System Attached with Tissue Engineered Periodontal-Ligament

Research Project

Project/Area Number 09557169
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Surgical dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OSAKI Teruhiko  Hiroshima University Scho.of Dentistry, Assistant Prof., 歯学部, 助手 (60243581)

Co-Investigator(Kenkyū-buntansha) AKAGAWA Yasumasa  Hiroshima University Scho.of Dentistry, Professor, 歯学部, 教授 (00127599)
OKAMOTO Tetsuji  Hiroshima University School of Dentistry, Professor, 歯学部, 教授 (00169153)
TORATANI Shigeaki  Hiroshima University Dental Hospital, Associate Prof., 歯学部・附属病院, 講師 (90172220)
Project Period (FY) 1997 – 1998
KeywordsPeriodontal-Ligament Cells / Epithelial rests of Malassez / Serum-Free Culture / FGF-7 / KGF / FGF receptor / Differentiation / Epithelial-Stromal Interaction
Research Abstract

In this study, we have developed a serum-free culture system of human periodontal ligament-derived epithelial cells (Epithelial rests of Malassez : ME) derived from Hertwig's epithelial root sheath.
The mitogenic effect of fibroblast growth factor (FGF) in the ME and human periodontal ligament-derived fibroblast cells (PLF) were investigated by serum-free growth assay. In addition, we demonstrated the expression of FGF receptor (FGFR) in the ME and the expression of FGF in the PLF by reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southem hybridization, ribonuclease protection assay (RPA) and immunohistochemical examinaijon.
Both FGF-1 and FGF-7/keratinocyte growth factor (KGF) stimulated cell growth of the ME.On the other hand, both FGF-1 and FGF-2 stimulated cell growth of the PLF.RT-PCR and PCR-Southem analysis revealed that the ME expressed FGFR2-(IIIb) mRNA but not mRNAs for FGFR1 -(IIIc), FGFR2-(IIIc), FGFR3-(IIIb), FGFR3-(IIIc) and FGFR4. An immunohistochemical. analysis of FGF-7/KGF revealed that immunoreactive FGF-7/KGF were detected predominantly in the cytoplasm of the. PLF cells. PCR-Southem and RPA also revealed that the PLF expressed FGF-7/KGF mR.NA.
These results suggest that the FGF-FGFR2-(IIIb) system plays an important role in the growth and differentiation of ME.In addition, endogenously produced FGFs work as paracrine interactive regulators of the growth and differentiation between ME and PLF.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Tetsuji OKAMOTO: "Growth and Differention of Periodontal Ligament-derived CELLS in serum-free defined culture" In Vitro Cellular Developmental Biol.33. 302-309 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuto HASHIMOTO: "Characteization of a cartilage-derived 66-kDa protein that binds to collages" Biochemica Et Biophysica Acta. 1355. 303-314 (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiharu TANAKA: "Molecular Diagnosis of Human Salivary gland tumors by Differential Exp." Tissue Culture Res.Commun.16. 207-213 (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiko MYOKEN: "Immunohistochemical Localization of FGF-1,FGF-2 and FGFR-1 in pleomorphic adenoma of the Salivary Gland" J.Oral Pathol.Med.26. 17-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji HIRAKI: "Local Signaling Mechanism in Chondrocytes Differentiation:A Role of FGF Signaling" Connective Tissue. 29. 219-226 (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] Miho FURUE: "Effects HGF and Activin A on the three dimensional growth and Morphogenesis of Rat Submandibular gland Cells." In Vitro Cell.Dev.Biol.34. 131-135 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, T., et al.: "Growth and differentiation of human periodontal ligament-derived cells in serum-free defined culture." In Vitro Cell.Dev.Biol.33. 302-309 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, K., et al.: "Characterization of a Cartilage-derived 66-KDa protein that Binds to Collagen" Biochemicaet Biophysica Acta. 1355. 303-314 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Y., et al.: "Moleculardiagnosis of human salivary gland tumors by differential expression of fibroblast growth factor receptor genes." Tissue Culture Res.Communications. 16. 207-212 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Myoken, Y., et al.: "Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and fibroblast growth factor receptor (FGFR-1) in pleomorphic adenoma of the salivary gland." J.Oral Pathol.Med.26. 17-22 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji HIRAKI,et al.: "Local Signaling Mechanism in Chondrogenic Differentiation : A Role of FGF Signaling." Connective Tissue. 29. 219-226 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, T., et al.: "Expression of fibroblast growth factor binding protein HBp17 in normal and tumor cells" In Vitro Cell.Dev.Biol.32. 69-71 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiriyama, T., et al.: "A Molecular Diagnosis of Crouzon Syndrome" Int.J.Oral & Maxillofacial Surgery. 26. 208 (1997)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, T., et al.: "A Case of Gingival Fibromatosis with Hypertrichosis" Int.J.Oral & Maxillofacial Surgery. 26. 209 (1997)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tani, R., et al.: "A Study of Cellular Immunity in Head and Neck Cancer Patients" Int.J.Oral & Maxillofacial Surgery. 26. 222 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Y., et al.: "Molecular Diagnosis and Gene Therapy of Salivary Gland Tumors." Int.J.Oral & Maxillofacial Surgery. 26. 262 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furue, M., et al.: "Effects of hepatocyte growth factor and activin A on the three dimensional growth and morphogenesis of rat submandibular gland epithelial cells embedded in collagen gels." In Vitro Cell.Dev.Biol.35 : 3. 131-135 (1999)

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      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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