1998 Fiscal Year Final Research Report Summary
Analysis of Neurosteroids and Its Application to Development of Anti-dementia Medicine
| Project/Area Number |
09557188
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
SHIMADA Kazutake Kanazawa University, Graduate School of Natural Science and Technology ; Professor, 自然科学研究科, 教授 (90004605)
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| Co-Investigator(Kenkyū-buntansha) |
HONMA Seijiro Teikoku Hormone Mfg Co. ; Head Research Director, 代謝部, 主席研究員
HIGASHI Tatsuya Kanazawa University, Faculty of Pharmaceutical Science ; Assistant, 薬学部, 助手 (90272963)
MITAMURA Kuniko Kanazawa University, Faculty of Pharmaceutical Sciences ; Assistant, 薬学部, 助手 (70242526)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Brain / Neurosteroid / Oxosteroid / Classical estrogen / Guaiacol estrogen / Stress / LC / MS / MS / GC / MS / MS |
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| Research Abstract |
The term neurosteroids applies to those steroids that are both synthesized in the nervous system, either de novo from cholesterol or from steroid hormone precursors, and that accumulate in the nervous system to levels that are at least in part independent of steroidogenic gland secretion rates. Neurosteroids consist of 17- or 20-oxosteroids and accumulate in brains as unconjugated form and their sulfates, fatty acid esters and sulfolipid conjugates.
An assay method for pregnenolone and its 3-stearate in brains has been developed using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS) operating in the positive ion mode. The method was applied to the determination of these steroids in the gray matters and olfactory bulbs of rat brains. Although pregnenolone in both regions of the rat brains increased more than five times after acute stress, its 3-stearate decreased after the stress.
The existence of classical and guaiacol estrogens in rat brains were also clarified using GC/MS^n. The usefulness of estrogen replacement therapy for dementia of the Alzheimer type and the existence of the estrogen receptor in brains have been reported, our data suggested that estrogen exists in brains as neurosteroids or neuroactive steroids.
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Research Products
(13 results)
