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1998 Fiscal Year Final Research Report Summary

Cloning and clinical application of telomerase cDNA

Research Project

Project/Area Number 09557195
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biological pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

IDE Toshinori  Hiroshima University Faculty of Medicine, Professor, 医学部, 教授 (60012746)

Co-Investigator(Kenkyū-buntansha) TAHARA Hidetoshi  Hiroshima University Faculty of Medicine, Research Associate, 医学部, 助手 (00271065)
Project Period (FY) 1997 – 1998
Keywordstelomere / telomerase / cellular lifespan / cellular senescence / cellular immortality / cancer diagnosis
Research Abstract

New findings of this project are 1) telomerase was partially purified from porcine testis to a few protein bands on acrylamidegel electrophoresis, but, since cDNA (hTERT) cloning of assumed catalytic subunit of telomerase has been reported by other reserchers (Aug. 1 997), we abandoned cloning project through protein, purification, 2) expression of hTERT mRNA was found to be correlated with presence 0f telomerase activity, 3) telomerase-negative human fibroblasts expressed telomerase activity after transfection with hTERT cDNA, 4) these telomerase positive human fibroblasts extended both telomere length and proliferative lifespan, but they did not immortalized, 5) telomerase-negative human fibroblasts transformed with SV40 T-antigen also expressed telomerase activity after. transfection with hTERT cDNA, 6) these telomerase-positive SV4O-transformed human fibroblasts extended both telomere length and proliferative lifespan, and they immortalized.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kitamoto,M: "Telomerase activity in precancerous hepatic nodules." Cancer. 85. 245-246 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tahara,H.: "Immuno-histochemical detection of human telomerase catalytic component,hTERT,in human colorectal tumor and non-tumor tissue sections." Oncogene. 18 in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,J.: "Telomerase activation by the catalytic component gene TRT in human primary fibroblasts and hepatocellular carcinomas." Nature Genetics. 18. 65-68 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirose,M.: "A new method to detect and measure telomerase activity by transcription-mediated amplification (TMA) and hybridization protection assay (HPA)." Clinical Chem.44. 2446-2452 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiki,T.: "Extra-Chromosomal telomerase repeat DNA in telomerase-negative immortalized cell lines." Biochem.Biophys.Res.Com.247. 765-772 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiyama,T.: "In situ mRNA hybridizaion technique for analysis of human telomerase RNA in gastric precancerous and cancerous lesions." Jpn.J.Cancer Res.89. 1099-1103 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui,W.: "Expression of telomerase catalytic component,telomerase reverse transcriptase,in humangastric carcinoma." Jpn.J.Cancer Res.89. 1187-1194 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakashio,R.: "Significance of telomerase activity in the diagnosis of small differentiated hepatocellualr carcinomas." Int.J.Cancer. 74. 141-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murakami,J.: "Telomerase activity in ovarian tumors." Cancer. 80. 1085-1092 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirose,M.: "A rapid,useful and quantitative method to measure telomerase activity by hybridization protection assay(HPA) connected with telomeric repeat amplification protocol(TRAP)." J.Cancer Res.Oncol.123. 337-344 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitamoto, M., et al.: "Telomerase activity in precancerous hepatic nodules." Cancer. 85. 245-246 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tahara, H., et al.: "Immuno-histochemical detection of Human telomerase catalytic component hTERT in human colorectal tumor and non-tumor tissue sections." Oncogene. 18(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama J., et al.: "Telomerase activation by the catalytic component gene TRT in human primary fibroblasts and hepatocellular carcinomas." Nature Genetics. 18. 65-68 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose, M., et al.: "A new method to detect and measure telomerase activity by transcription-mediated amplification (TMA) and hybridization protection assay (HPA)." Clinical Chem.44. 2446-2452 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiki, T., et al.: "Extra-Chromosomal telomerase repeat DNA in telomerase-negative immortalized cell lines." Biochem.Biophys.Res.Com.24. 765-772 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiyama, T., et al.: "In situ mRNA hybridization technique for analysis of human telomerase RNA in gastric pre-cancerous and cancerous lesions." Jpn.J.Cancer Res.89. 1099-1103 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui, W., et al.: "Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinoma." Jpn.J.Cancer Res.89. 1187-1194 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakashio, R., et al.: "Significance of telomerase activity in the diagnosis of small differentiated hepatocellular carcinomas." Int.J.Cancer. 74. 141-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami, J., et al.: "Telomerase activity in ovarian tumors." Cancer. 80. 1085-1092 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose, M., et al.: "A rapid useful and quantitative method to measure telomerase activity by hybridization protection assay (HPA) connected with telomeric repeat amplificatior protocol (TRAP)." J.Cancer Res.Oncol.123. 337-344 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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