1998 Fiscal Year Final Research Report Summary
Molecular design of inhibitors for DNA replication in Escherichia coli
| Project/Area Number |
09557198
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SEKIMIZU Kazuhisa Faculty of Pharmaceutical Sciences, Kyushu University, Professor, 薬学部, 教授 (90126095)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Takeyoshi Faculty of Pharmaceutical Sciences, Kyushu University, Associate Professor, 薬学部, 助教授 (40037586)
SASAKI Shigeki Faculty of Pharmaceutical Sciences, Kyushu University, Associate Professor, 薬学部, 助教授 (10170672)
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| Project Period (FY) |
1997 – 1998
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| Keywords | DnaA protein / Esherichia coli / DNA replication / DNA polymerase III / the dnaN genen / oriC replication |
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| Research Abstract |
Initiation of DNA replication in cells is negatively or positively regulated by various factors. We tried to identify those regulating factors by using biochemical and genetic approaches. In crude extacts of E.coli, we identified an activity (RIDA, regulatory inhibition of DnaA protein) which stimulates hydrolysis of ATP bound to DnaA protein, the initiator of DNA replication in E coli. Purification of this factor demonstrated that the beta subunit of DNA polymerase III holoenzyme is responsible for this activity. In a mutant cells of the dnaN gene, which encodes the beta subunit, the ADP-binding form of DnaA protein, which is inactive for DNA replicatoin, was shown to be accumulated. The result confirms that the beta subunit stimulates hydrolysis of ATP bound to DnaA protein. This is the first report indicating that a subunit of DNA polymerase, which is responsible for catalytic reaction of DNA synthesis, negatively regulates initiation of DNA replication.
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[Publications] Mizushima T., Takaki, T., Kubota, T., Tsuchiya, T., Miki, T., Katayama, T., and Sekimizu, K.: "Site-directed mutational analysis for the ATP binding of DnaA protein." J.Biol.Chem.273. 20847-20851 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kurokawa, K., Mizushima, T., Kubota, T., Tsuchiya, T., Katayama, T., and Sekimizu, K.: "A stimulation factor for hydrolysis of ATP bound to DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli" Biochem.Biophys.Res.Commun.243. 90-95 (1998)
Description
「研究成果報告書概要(欧文)」より
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