1998 Fiscal Year Final Research Report Summary
Functional Conversion and Artificial Repressor of Zinc Finger Proteins
| Project/Area Number |
09557200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
医薬分子機能学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SUGIURA Yukio Kyoto Univ., Inst.Chem.Res., Prof., 化学研究所, 教授 (40025698)
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| Co-Investigator(Kenkyū-buntansha) |
OKUNO Yasushi Kyoto Univ., Inst.Chem.Ris., Instructor, 化学研究所, 教務職員 (20283666)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Zinc finger / DNA binding / Base recognition / Artificial repressor / Transcription factor / Molecular design / Genomu information / Functional conversion |
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| Research Abstract |
DNA recognition is a very important phenomenon in biology and chemistry, and the molecular basis of the sequence-specific DNA binding is the subject of investigations aimed at the rational design of artificial repressors. Zinc finger libraries with different DNA-binding specificites based on a one-finger-three-nuclestide code have been developed successfully. Newly designed zinc finger protein containing nine Cys_2His_2 type motifs were manipulated, and the DNA binding property of this multi finger protein was compared with that of nation three zinc finger protein Sp1. Evidently, the footprinting analysis demonstrated that the nine finger protein binds at least 27 contiguous base pairs of DNA sequence. Six zinc finger proteins induced sequence-specific DNA bending were also designed and a bent in the DNA was determined by electrophoretic methods. Such multi zinc finger protein may be useful as genome-specific transcriptional swiches in future.
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