| Co-Investigator(Kenkyū-buntansha) |
YONEKAWA Hiromichi The Tokyo Metropolitan Organization of Medical Science, Tokyo Metropolitan Institute of Medical Science, Vice president, 東京都臨床医学総合研究所, 副所長 (30142110)
KARASUYAMA Hajime The Tokyo Metropolitan Organization of Medical Science, Tokyo Metropolitan Institute of Medical Science, Department of Tumor Immunology, Researcher, 東京都臨床医学総合研究所, 研究員 (60195013)
|
|---|
| Research Abstract |
The aim of the project is to establish model mice for auto-immune neuropathy by transfection with anti-carbohydrate antibody genes. A hybridoma producing a monoclonal antibody, which reacts specifically with b-pathway gangliosides such as GD3, GD2, GD1b, GT1b, and GQ1b, but not with any other gangliosides or neutral glycosphingolipids was selected among a series of hybridomas secreting monoclonal antibodies specific for carbohydrate chains, that were established in our laboratory. We generated, at first, transgenic mice having high antibody titer of IgM to the ganglioides. These mice appeared to be healthy and exhibited no significant pathological symptoms at 1-year-old. Next, we tried to induce neuropathy by immunizing these transgenic mice with a number of adjuvants including LPS and cytokines. However, they showed no significant symptoms. These results suggest that only high antibody titer to the gangliosides in sera is not enough for inducing neuronal symptoms. Further study will be needed for analyzing the mechanisms of the neuropathy. At present, we are producing transgenic mice having high antibody titer of IgG.
|
