1999 Fiscal Year Final Research Report Summary
Development of a micro tensile tester for the in-process monitoring of cell structure
| Project/Area Number |
09558109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MATSUMOTO Takeo Grad Sch Engng, Tohoku Univ, Assoc Prof, 大学院・工学研究科, 助教授 (30209639)
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| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Noriyuki Grad Sch Engng, Tohoku Univ, Res Assoc, 医用電子技術科, 講師 (20250681)
OHASHI Toshiro Grad Sch Engng, Tohoku Univ, Res Assoc, 大学院・工学研究科, 助手 (30270812)
SATO Masaaki Grad Sch Engng, Tohoku Univ, Professor, 大学院・工学研究科, 教授 (30111371)
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| Project Period (FY) |
1997 – 1999
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| Keywords | cellular mechanics / tensile test / elastic modulus / viscoelasticity / smooth muscle cell / mechanical property / phenotype |
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| Research Abstract |
In this 3-year study, we have developed a micro tensile tester to measure tensile properties of a single isolated cell and to observe change in intracellular structure during stretching simultaneously. Both ends of the cell were aspirated with glass micropipettes (3-15mm in I.D.) coated with cellular adhesive (Cell-Tak), and one pipette was moved with an electrical manipulator (MMS-77, Shimadzu) to stretch the cell under a microscope (BX50WI, Olympus). The force applied to the cell was measured by the deflection of a cantilever part of the other pipette. To obtain its clear image, the cell was observed with water immersion objectives under the upright microscope. To make cell manipulation easier, the stage of the microscope was fixed and the objectives were moved to adjust the focus. Bovine aortic smooth muscle cells (BASMs) obtained by the explant method (P6-7) and rat aortic smooth muscle cells (RASMs) obtained by the enzymatic dispersion method were successfully stretched with the tester under a physiological salt solution at 37℃ to obtain their tensile properties. Tensile tests of BASMs at various stretch rate show that initial elastic modulus of the cell have a significant positive correlation with the strain rate over 4 %/s. Elastic modulus of BASMs and RASMs were about 3 kPa and about 12kPa, respectively under the strain rate of 0.2-4 %/s. Due to the difference in the cell preparation method, BASMs and RASMs are considered to show synthetic and contractile phenotype, respectively. Contractile cells contain much more contractile proteins than synthetic cells. Contractile proteins may be stiffer than cytoplasm. Contractile cells may thus be stiffer than synthetic cells. The difference in the mechanical properties of BASMs and RASMs in the present study may be due to the difference in the phenotype of the two cell specimens.
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