1998 Fiscal Year Final Research Report Summary
Development of micromachined biosensor system for biomedical application
| Project/Area Number |
09559007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | Japan Advanced Institute of Science and Technology Hokuriku |
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Principal Investigator |
TAMIYA Eiiti JAIST,School of Materials Science, Professor, 材料科学研究科, 教授 (60179893)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKA Eiji Shibuya Kogyo co., Ltd, Mechatronics Production Div.Researcher, メカトロ生産本部医療器設計課, 研究員
SAKAGUTI Tisifumi JAIST,School of Materials Science, Associate, 材料科学研究科, 助手 (10272999)
MURAKAMI Yuuji JAIST,School of Materials Science, Associate, 材料科学研究科, 助手 (70272995)
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| Project Period (FY) |
1997 – 1998
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| Keywords | biosensor / blood dialysis / on-line monitoring / micromachine / immobilized enzyme / blood urea nitrogen (BUN) / ion sensitive field effect transistor (ISFET) |
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| Research Abstract |
Development of micromachined biosensor system for biomedical application
A urine sensor at a waste drain of an artificial blood dialysis system was deveIoped for the continuous monitoring of urine. Urease was dropped on an ISFET, and cross-linked by glutaraldehyde. The sensor system was also equipped in the waste drain of the blood dialysis system using artificial blood. The results showed the usefulness of the sensing system for the urea monitoring during dialysis.
We also performed the fundamental research of a SPV device, which is useful to construct an integrated pH-sensing device. BOD sensor as a typical microbial sensor was constructed with the SPV device. Usually BOD sensor employs an oxygen electrode as a transducer. However, we succeeded to fabricate a biosensor that measures values related to BOD.In principle, when a sample solution was added, microorganism on a SPV device produces acidic compounds, and the device measures it.
Furthermore, we proposed a novel method to immobilize various biomaterials on densely arrayed transducers at once to construct integrated multifunctional biosensing system. Microfabricated particles immobilized with enzymes for biosensor application were randomly arranged on a chip by fluidic self-assembly method. As the short-range force required in self-assembly, we employed gravity. A bead immobilized with enzyme fell into microchamber, After the immobilization of biomaterial on the microparticle, the suspension of microparticles were added on the chip to be ordered by random fluidic self-assembly. The immobilization of biomaterial was evaluated by chemiluminescence method catalyzed by immobilized enzymes.
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