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1998 Fiscal Year Final Research Report Summary

Analysis of three-dimensional structure of ion channel peptides bound to phospholipid bilayers by high resolution solid state NMR

Research Project

Project/Area Number 09640612
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionHimeji Institute of Technology

Principal Investigator

NAITO Akira  Department of Life Science, Himeji Institute of Technology Associate Professor, 理学部, 助教授 (80172245)

Co-Investigator(Kenkyū-buntansha) TUZI Satoru  Department of Life Science, Himeji Institute of Technology Assistant Professor, 理学部, 助手 (60227387)
Project Period (FY) 1997 – 1998
KeywordsIon channel / Enkephalin / Melittin / Interatomic distance / High resolution solid state NMR / Three-dimensional structure / Phospholipid bilayer / Magnetically oriented membrane
Research Abstract

In this research project, we have studied to achieve a new approach to determine the three-dimensional structure and orientation of membrane bound ion channel peptides. Following results were obtained in this research project.
(1) The three-dimensional structure of [ィイD113ィエD1C, ィイD115ィエD1N]-labeled Leu-enkephalin (tyr-Gly-Gly-Phe-Leu) dihydrate crystals was determined on the basis of six sets of accurately determined ィイD113ィエD1C-ィイD115ィエD1N interatomic distances by rotational echo double resonance (REDOR) and some additional constraints from ィイD113ィエD1C chemical shifts.
(2) Interatomic distances of isotopically doubly labeled N-Acetyl-Pro-Gly-Phe crystals, obtained from rotational echo double resonance (REDOR) experiments, were analyzed as a three-spin system in which observed nuclei are simultaneously coupled with isotopically labeled intra- and intermolecular heteronuclei. In particular, the intermolecular dipolar interaction was examined to obtain a clue as to the relative orientation of a given molecule to the surrounding neighbors. We have developed a systematic procedure to determine both the intra- and intermolecular interatomic distances and the angle between three two interatomic vectors.
(3) The conformation and dynamics of melittin bound to the dimyristoylphophatidylcholin (DMPC) bilayer and the magnetic orientation in the lipid bilayer systems were investigated by solid-state ィイD131ィエD1P and ィイD113ィエD1C NMR spectroscopy. Using ィイD131ィエD1P NMR, it was found that melittin-DMPC bilayer system forms magnetically oriented elongated vesicles with the long axis parallel to the magnetic field above the liquid crystalline-gel phase transition temperature. ィイD113ィエD1C NMR spectra were observed on the ィイD113ィエD1C labeled melittin bound to the lipid bilayers. Finally, it was found that melittin adopts a transmembrane a-helix whose average axis is parallel to the bilayer normal.

  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] A. Gil: "A^<13>CNMR Study on the Conformation and Dynamical Properties of a Cereal Strorage Protein, C-Hordein, and Its Model Peptides"Biopolymers. 41. 589-300 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Nakatsuji: "Preparation and prorerties of 2-(o-halophenyl)-a-nitonyl nitroxides"Mol. Cryst. Liq. Cryst.. 306. 279-284 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Yamaguchi: "Stability of C-Terminal-Helical Domain of Bacteriorhodopsin Protruding from the Membrane Surface as Studied by High-resolution Solid-state ^<13>CNMR"J. Biochem. (Tokyo). 123. 78-86 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Saito: "Conformation and Dynamics of Membrane Proteins and Biologically Active Peptides as Studied by High-resolution Solid-state ^<13>CNMR"J. Mol. Structure. 441. 137-148 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Naito: "Backbone Dynamics of Polycrystalline Peptides Studied by Measurements of ^<15>NNMR Lineshpes and ^<13>C Transverse Relaxation Times"J. Mol. Structure. 441. 231-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. K. Dioumaev: "Existence of a Proton Transfer Chain in Bacteriorhodopsin : Participation of Glu-194 in the Release of Protons to the Extracellular Surface"Biochemistry. 37. 2496-2506 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Kamihira: "A High-resolution Solid-state ^<13>C and ^<15>NMNR Study on Crystalline Leu-and Met-enkephalins : Distinction lf Polymorohs, Backbone Dynamics and Local Conformational Rearrangements Induced by Dehydration or Freezing Motions of Bound Solvent Molecules"J. Phys. Chem. B. 102. 2826-2834 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Saito: "Empirical versus Nonempirical Evaluation of Secondary Structure of Fibrous and Membrane Proteins by Solid-state NMR : A Practical Approach"Annu. Rep. NMR Spectrosc.. 36. 79-121 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Naito: "Dipolar Interaction and Interatomic Distances"Physical and Theoretical Chemistry. 84. 22-49 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Saito: "Polysaccharides and Biological Systems"Physical and Theoretical Chemistry. 84. 891-921 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Iwasa: "^<19>F-NMR and UV-Vis Absorption Spectroscopic Studies of Fluorinated Octopus Rhodopsin and Its Photoproducts"J. Phys. Chem. A.. 102. 5602-5610 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Nakatsuji: "2-(2'-Halophenyl)-a-nitronyl nitroxides"New J. Chem.. 102. 275-280 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Nishimura: "Determination of the Three-Dimensional Structre of Crystalline Leu-Enkephalin Dihydrate Based on Accurately Determined Six Sets of Interatomic Distances from ^<13>C-REDOR NMR and the Conformation-Dependent ^<13>C Chemical Shifts"J. Phys. Chem. B.. 102. 7476-7483 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Tanio: "Evidence of Local Conformational Fluctuations and Changes in Bacteriorhodopsin, Dependent on Lipids, Detergents and Tremeric Structure, as Studied by^<13>CNMR"Biochim. Biophys. Acta. 1375. 84-92 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Tuzi: "Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by ^<13>CNMR"Biophys.J.. 76. 1523-1531 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Naito: "Conformation and bynamics of melittin bound to magnetically oriented lipid vilayers by solid-state ^<31>P and ^<13>CNMR spectroscopy"Biophys. J.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Gil: "A ィイD113ィエD1C NMR Study on the Conformation and Dynamical Properties of a Cereal Storage Protein, C-Hordein, and Its Model Peptides"Biopolymers. 41. 289-300 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Nakatsuji: "Preparation and properties of 2-(o-halophenyl)-a-nitonyl nitroxides"Mol. Cryst. Liq. Cryst.. 306. 279-284 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Yamaguchi: "Stability of C-Terminal -Helical Domain of Bacteriorhodopsin Protruding from the Membrane Surface as Studied by High-resolution Solid-state ィイD113ィエD1C NMR"J. Biochem. (Tokyo). 123. 78-86 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Saito: "Conformation and Dynamics of Membrane Proteins and Biologically Active Peptides as Studied by High -resolution Solid-state ィイD113ィエD1C NMR"J. Mol. Structure. 441. 137-148 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Naito: "Backbone Dynamics of Polycrystalline Peptides Studied by Measurements of ィイD115ィエD1N NMR Lineshpes and ィイD113ィエD1C Transverse Relaxation Times"J. Mol. Structure. 441. 231-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. K. Dioumaev: "Existence of a Proton Transfer Chain in Bacteriorhodopsin : Participation of Glu-194 in the Release of Protons to the Extracellular Surface"Biochemistry. 37. 2496-2506 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Kamihira: "A High-resolution Solid-state ィイD113ィエD1C and ィイD115ィエD1N NMR Study on Crystalline Leu- and Met-enkephalins : Distinction of Polymorphs, Backbone Dynamics and Local Conformational Rearrangements Induced by Dehydration or Freezing Motions of Bound Solvent Molecules"J. Phys. Chem. B.. 102. 2826-2834 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Saito: "Empirical versus Nonempirical Evaluation of Secondary Structure of Fibrous and Membrane Proteins by Solid-state NMR : A Practical Approach"Annu. Rep. NMR Spectrosc.. 36. 79-121 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Naito: "Dipolar Interaction and Interatomic Distances"Physical and Theoretical Chemistry. 84. 22-49 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Saito: "Polysaccharides and Biological Systems"Physical and Theoretical Chemistry. 84. 891-921 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Iwasa: "ィイD119ィエD1F-NMR and UV-Vis Absorption Spectroscopic Studies of Fluorinated Octopus Rhodopsin and Its Photoproducts"J. Phys. Chem. A.. 102. 5602-5610 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Nakatsuji: "2-(2'-Halophenyl)-a-nitronyl nitroxides"New J. Chem.. 275-280 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nishimura: "Determination of the Three-Dimensional Structure of Crystalline Leu-Enkephalin Dihydrate Based on Accurately Determined Six Sets of Interatomic Distances from ィイD113ィエD1C-REDOR NMR and the Conformation -Dependent ィイD113ィエD1C Chemical Shifts"J. Phys. Chem. B. 102. 7476-7483 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Tanio: "Evidence of Local Conformational Fluctuations and Changes in Bacteriorhodopsin, Dependent on Lipids, Detergents and Trimeric Structure, as Studied by ィイD113ィエD1C NMR"Biochem. Biophys. Acta. 1375. 84-92 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Tuzi: "Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by ィイD113ィエD1C NMR"Biophys. J.. 76. 1523-1531 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Naito: "Conformation and dynamics of melittin bound to magnetically oriented lipid bilayers by solid-state ィイD131ィエD1P and ィイD113ィエD1C NMR spectroscopy"Biophys. J.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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