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1998 Fiscal Year Final Research Report Summary

Formation of Porphyrin-metal Complex Catalyzed by Single Strand DNA

Research Project

Project/Area Number 09640664
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Inorganic chemistry
Research InstitutionKyoto Institute of Technology

Principal Investigator

TAJIMA Kunihiko  Kyoto Institute of Technology, polymer Science and Engineering, Associate Prof., 繊維学部, 助教授 (50163457)

Project Period (FY) 1997 – 1998
KeywordsDNA / DNA-enzyme / heme / DNA-heme interaction / heme-metallation / 銅錯体 / ボルフィリン
Research Abstract

In this study, the structural study on porphyrin-DNA interactions was performed in order to clarify whether it is possible for single-stranded DNAs to fold to create specific binding pockets for anionic porphyrins, which do not naturally bind to nucleic acids.
The kinetics of the metallation of Cu^<2+> into MPIX was first examined in the presence and absence of PS5.ST1 (dTCGTGGGTCATTGTGGGTGGGTGTGGCTGGTCC) and other G-rich oligomers by HPLC.The results were almost identical to that as previously reported (3).
CD spectroscopy has been used to distinguish between parallel and antiparallel G-quadruplexes (4,5) The former quadruplexes exhibit a positive CD band around 260 nm and a negative CD band at 240 nm. The latter quadruplexes exhibit a positive CD band around 290 nm and a weaker negative band at 265 nm. CD spectroscopy was carried out for PS5.ST 1 (25 muM) at various temperatures in the absence and presence of K^+ ion. In the absence of K^+, this sample showed two positive peaks at 265 and 285 nm at 0゚C, while at increasing temperatures, one at 265 nm disappeared, and the other at 285 nm decreased. Temperature-dependent CD spectroscopy was also performed for PS5.ST1 in the presence of K^+ ion in the temperature range of 0-80゚C.Both the positive peaks appeared below 25゚C, and the intensity of the peak at 265 nm was much stronger than that in the absence of K^+ ion. These results indicate that even in the absence of K^+ ion, PS5.ST1 takes an ordered structure, and that the addition of K^+ ion induces components of parallel G-quadruplexes.
^1HNMR measurements were carried out for PS5.ST1 in the absence and presence of K^+ ion. The imino proton signals are an indicator of symmetry and stoichiometry. The NMR spectra show some change in the chemical shifts of the peaks, which may correspond with the change in the CD spectra.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Kenji Kanaori: "H Nuclear Magnetic Resonace Study on Equilibrium between Two Four-stranded Solution Conformations of Short d(CnT)" Biochemistry. 37-37. 12979-12986 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshinori Suzuki: "Misincorporation of 2Af-Deoxyoxanosine-5Aftriphosphate by DNA Polymerases and Its Impulication for Mutagenesis" Biochemistry. 37-33. 11592-11598 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Tajima: "Formation of Porphyrin-metal Complex Catalyzed by Single Strand DNA" Nucleic Acids Symposium Series. 39. 169-170 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Tajima: "Spectroscopic and ESR studies of p-p stacked binuclear copper(II) complexes of w-wAf-bis(pyridine-2-carboxyamide)alkanes in acidic soluiton" Inorganica Chimica Acta. 283. 61-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Tajima: "Crystallographic and ESR studies of stacked binuclear copper(II) complexes of 1,3-bis(quinoline-2-carboxyamide-and 1,3-bis(isoquinoline-2-carboxyamide)propane" Acta Crystallographica Section C. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Tajima: "〔1,3-bis(pyridine-2-carboxyamidato)propane〕paradium(II)and nickel(II)mono-hydrate" Inorganica Chimica Acta. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakamur, T.Ikeue, H.Fujii, T.Yoshimura, K.Tajima: "Electron Configuration and Spin Distribution in Sow-spin (meso-tetra-alkylporphyrin Complexes Carriying One or Two Orientationally Fixed Imidazole logand" Inorg.Chem. 37. 2405-2414 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Oka, K.Tajima, and H,Sakurai: "A Chemical Model of Tryptophan 2,3-Dioxygenase : Reactivity and Formation of a Reactive Intermadiate in the Dioxygenase" Chem.Pharm.Bull.46. 377-383 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kanaori, K.Yokoyama, K.Tajima, N.Yamamoto, T.Okamoto, and K.Makino: "Interaction of Pyrylium Dye with Self-complementary DNA Oligomer as Studied by 1H-NMR Spectroscopy" Nucleotide & Nucleotides. 17. 603-611 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tajima, S.Ishikawa, K.Kanaori, K.Makino: "Formation of Porphyrin-metal Complex Catalyzed by Single Strand DNA" Nucleic Acids Symposium series. 39. 169-170 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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