| Research Abstract |
As for the insertional mutagenesis tool, T-DNA and transposon such as Ac or Spin derived from maize were used frequently in plants. Although Arabidopsis, that is most major model plant, has its intrinsic transposon Tag1, no tool was developed from Tag1. In this project, we found 1/3 size homologue of Tag1 that was thought to be in Ler., but not to be in Col. ecotypes of Arabidopsis. Interestingly, many copy of this homologue was found over the genome sequence data of Col.. More over, we found this homologue is distributed over wide plant kingdom including gymnosperm and angiosperm. We started to test to obtain mutant lines with the construct including Tag1 and antibiotics resistant marker. Belonging this project, we developed a unique technique that collect transcripts derived from target locus. We also made high density RFLP map that will help identification of the location of insertional mutation. Coupling of these two technique, 'actually insertional mutants were obtained at specific locus. On the other hand, we analyzed phenyl alanine ammonia lyase gene and its induction with methyl jasmonate or elicitor, and also genes for kinases. These induction system or genes will be used as evaluating target of novel tools for insertional mutagenesis.
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