• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Genetic and molecular analyzes of bacterial flagellum which acts as a transcription-controlling apparatus

Research Project

Project/Area Number 09640737
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KUTSUKAKE Kazuhiro  Hiroshima University Faculty of Applied Biological Science Professor, 生物生産学部, 教授 (90143362)

Project Period (FY) 1997 – 1998
KeywordsBacterial flagella / Transcriptional control / Signal transduction / Anti-sigma factor / Flagellar hook / Protein export / Export switch / Hook length control
Research Abstract

During flagellar morphogenesis in Salmonella typhirnurium, the genes involved in filament assembly are expressed fully only after completion of hook assembly. This coupling of gene expression to morphogenesis is achieved by exporting the flagellum-specific anti-sigma factor, FlgM, out of the cell through the mature hook-basal body structure. Therefore, the flagellum-specific export apparatus must be able to sense the assembly state of the flagellar structure and to turn on FlgM export at the specific stage of hook assembly. In this study, this export switching machinery was dissected at a molecular level. The main conclusions are as follows. (1) The export gate for FlgM is double locked by FlhB and RflH proteins before completion of hook assembly. (2) These locks are opened in the mutants which produce abnormally elongated rods lacking hooks. This indicates that the monitor system of hook length measures the length of the rod plus the hook. (3) The FlgM gate is also involved in the export control of the late flagellar proteins, FlgK, FlgL, FliD, and FliC.FlhB is also responsible for shutting-off process of the export of hook protein after completion of hook assembly. (4) Mutants in which the RflH lock is opened constitutively were isolated. Genetic analysis of these mutants suggested that at least two proteins are involved in the opening process of the RflH lock. (5) The FlhB lock is opened at the early stage of hook assembly, whereas the RflH lock is opened upon completion of hook assembly.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kutsukake,K.: "Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis" Journal of Bacteriology. 179(4). 1268-1273 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kutsukake,K.: "Autogenous and global control of the flagellar master operon,flhD,in Salmonella typhimurium" Molecular and General Genetics. 254(4). 440-448 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ide,N.: "Identification of a novel Escherichia coli gene whose expression is dependent on the flagellum-specific sigma factor,FliA,but dispensable for motility development" Gene. 199(1). 19-23 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nambu,T.: "Peptidoglycan-hydrolyzing activity of the FlgJ protein,essential for flagellar rod formation in Salmonella typhimurium" Journal of Bacteriology. 181(5). 1555-1561 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikebe,T.: "Structure and expression of the fliA operon of Salmonella typhimurium" Microbiology. 145印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamino,T.: "Substrate specificity switching of the flagellum-specific export apparatus during flagellar morphogenesis in Salmonella typhimurium" Bioscience,Biotechnology,and Biochemistry. 63印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 沓掛和弘(分担執筆): "分子細胞生物学辞典" 東京化学同人(村松正実他編集), 1025 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 沓掛和弘(分担執筆): "化学と生物 35巻" 学会出版センター(日本農芸化学会編集), 884 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kutsukake, k.: "Hook-length control of the export-switching machinery involves a double-locked gate in salmonella typhimurium flagellar morphogenesis" Journal of Bacteriology. 179(4). 1268-1273 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kutsukake, K.: "Autogenous and global control of the flagellar master operon, flhD,in Salmonella typhimurium" Molecular and General Genetics. 254(4). 440-448 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ide, N.and Kutsukake, K.: "Identification of a novel Escherichia coli gene whose expression is dependent on the flagellum-specific sigma factor, FliA,but dispensable for motility development" Gene. 199(1). 19-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nambu, T., Minamino, T., Macnab, R.M., and Kutsukake, K.: "Peptidoglycan-hydrolyzing activity of the FlgJ protein essential for flagellar rod formation in Salmonella typhimurium" Journal of Bacteriology. 181(5). 1555-1561 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikebe, T., Iyoda, S., and Kutsukake, K.: "Structure and expression of the fliA operon of Salmonella typhimurium" Microbiology. 145 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamino, T., Doi, H., and Kutsukake, K.: "Substrate specificity switching of the flagellum-specific export apparatus during flagellar morphogenesis in Salmonella typhimurium" Bioscience, Biotechnology, and Biochemistry. 63 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi