1998 Fiscal Year Final Research Report Summary
The role of cotA gene in proton extrusion of Synechocystis PCC6803
| Project/Area Number |
09640767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Nagoya University |
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Principal Investigator |
OGAWA Teruo Nagoya University Bioscience Center, Professor, 生物分子応答研究センター, 教授 (80087593)
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| Project Period (FY) |
1997 – 1998
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| Keywords | cyanobacteria / proton extrusion / Synechocystis / Synechococcus / pxcA / cotA / cytoplasmic membrane / cemA |
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| Research Abstract |
Light-induced proton extrusion has been observed with various strains of cyanobacteria. We found that pxcA (formerly named as cotA) complemented a Synechocystis mutant unable to grow at acidic pH and that inactivation of this gene abolished the activity of light-induced proton extrusion. PxcA genes of Synechocystis PCC6803 and Synechococcus PCC7942 encode proteins of 440 and 427 amino acids, respectively, that showed significant sequence homology to a protein in the inner envelope membrane of chloroplasts encoded by cemA in chloroplast genome. We have demonstrated that PxcA is localized in the cytoplasmic membrane. The pxcA-inactivated mutant was unable to grow at acidic pHs or at low Na^+ concentrations. The activities of CO_2 uptake and NO_3 uptake by the mutant were inhibited under these conditions. There are two types of CemA/PxcA.CemA in chloroplasts of higher plants consists of 229 - 231 amino acids whereas CemA/PxcA in liverwort, Chlamydomonas and cyanobacteria are much larger and consist of 421-500 amino acids. Both short and long types of CemA/PxcA contain 4 membrane spanning domains. Phylogenetic tree for PxcA of cyanobacteria and CemA of plants and Chlamydomonas suggested that PxcA and CemA have the same ancestor.
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[Publications] Sonoda,M.,Katoh,H.,Katoh,A.,Ohkawa,H.,Vermaas,W.,Ogawa,T.: "Structure and function of cemA homologue (pxcA) in cyanobacteria." The Chloroplast: From Molecular Biology to Biotechnology (ed.Akuyunoglou),. 印刷中. (1999)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sonoda, M., Kitano, K., Katoh, A., Katoh, H., Ohkawa, H.and Ogawa, T.: "Size of cotA and identification of the gene product in Synechocystis sp.strain PCCC6803." Journal of Bacteriology. 179. 3845-3850 (1997)
Description
「研究成果報告書概要(欧文)」より
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