• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Plant cysteine protease: multiple posttranslational processing and intracellular transport

Research Project

Project/Area Number 09640776
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionTOKYO METOROPOLITAN UNIVERSITY

Principal Investigator

MINAMIKAWA Takao  Dept. of Biol. Science Professor, 理学研究科, 教授 (30087001)

Project Period (FY) 1997 – 1999
Keywordscysteine protease / endopeptidase / intracellular transport / Vigna mungo / seed germination / posttranslational processing / endoplasmic reticulum / vacuole
Research Abstract

A vacuolar cysteine proteinase, designated SH-EP, is expressed in the cotyledon of germinated Vigna mungo seeds, and is responsible for the degradation of storage proteins. SH-EP is a characteristic vacuolar proteinase possessing a carboxy-terminal endoplasmic reticulum (ER) retention secuence, KDEL. In the present work, immunocytochemical analysis of the cotyledon cells of V. mungo seeds was performed using seven kinds of antibodies to identify the intracellular transport of SH-EP from ER to protein storage vacuoles. A proform of SH-EP synthesized in ER accumulated at the edge of middle regions of ER where the transport vesicle was formed. The vesicle containing a large amount of proSH-EP, termed KV, budded off from ER, bypassed the Golgi complex and was sorted to protein storage vacuoles. This massive transport of SH-EP via KV was thought to mediate dynamic protein mobilization in the cotyledon cells of germinated seeds. We discuss the possibilities that the KDEL sequence of KDEL-tailed vacuolar cysteine proteinases functions as an accumulation signal at ER and that the mass transport of the proteinases by ER-derived KV-like vesicle is involved in the protein mobilization of plants.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Okamoto, T., Minamikawa, T.: "A vacuolar cysteine endopeptidase (SH-EP) that digests seed storage globulin : characterization, regulation of gene expression, and posttranslational processing"J. Plant Physiol.. 152. 675-682 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, T., Minamikawa, T.: "Molecular cloning and characterization of Vigna mungo processing enzyme 1 (VmPE-1), an asparaginyl endopepetidases"Plant Mol. Biol.. 39. 63-73 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, T., Minamikawa, T. et al.: "Posttranslational removal of the carboxyl-terminal KDEL of the cysteine protease SH-EP occurs prior to maturation of the enzyme"J. Biol. Chem.. 274. 11390-11398 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosokawa, T., Okamoto, T., Minamikawa, T.: "Characterization of serine endopeptidases in cotyledons of germinated Vigna mungo seeds"J. Plant Res.. 112. 217-221 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, T., Yuki, A., Mitsuhashi, N., Minamikawa, T.: "Asparaginyl endopeptidase (VmPE-1) and autocatalytic processing sysnergistically activate the vacolar cysteine proteinase (SH-EP)"Eur. J. Biochem.. 264. 223-232 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyooka, K., Okamoto, T. Minamikawa, T.: "Mass transport of proform of a cysteine proteinase (SH-EP) to protein storage vacuoles by ER-derived vescle is involved in protein mobilization in germinating seeds"J. Cell Biol.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, T., Minamikawa, T.: "A vacuolar cysteine endo peptidase (SH-EP) that digests seed storage globulin: characterization, regulation of gene expression, and posttranslational processing"J. Plant Physiol.. 152. 675-682 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, T., Minamikawa, T.: "Molecular cloning and characterization of Vigna mungo processing enzyme l (VmPE-l), an asparaginyl endopepetidase possibly involved in post-translational processing of a vacuolar cysteine endopeptidase (SH-EP)"Plant Mol. Biol.. 39. 63-73 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, T., Minamikawa, T., Edward, G., Vakharia, V., Herman, E.: "Posttranslational removal of the carboxylterminal KDEL of the cysteine protease SH-EP occurs prior to maturation of the enzyme"J. Biol. Chem.. 274. 11390-11398 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosokawa, T., Okamoto, T., Minamikawa, T.: "Characterization of serine endopeptidases in cotyledons of germinated Vigna mungo seeds"J. Plant Res.. 112. 217-221 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, T., Yuki, A., Mitsuhashi, N., Minamikawa, T.: "Asparaginyl endopeptidase (VmPE-l) and autocatalytic processing synergistically activate the vacuolar cysteine proteinase (SH-EP)"Eur. J. Biochem.. 264. 223-232 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyooka, K., Okamoto, T., Minamikawa, T.: "Mass transport of proform of a cysteine proteinase (SH-EP) to protein storage vacuoles by ER-derived vesicle is involved in protein mobilization in germinating seeds"J. Cell Biol.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi